Figure 1.

Organization of the hmw gene cluster (17). The genes encoding P30, HMW3, HMW1, and ribosomal protein S4 are indicated. The remaining ORFs have no clear identity to genes of known function and are identified by size (kDa) of their deduced products. Transcriptional start sites (P.L.P. and D.C.K., unpublished results) are indicated by arrows, and predicted terminators are designated by circles. B, BamHI; E, EcoRI; H, HindIII; K, KpnI; P, PstI. The (E) designates an EcoRI site engineered in the original cloning of hmw3 by ligation of a linker to sheared mycoplasma DNA (18).