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Pulse–chase analysis of HMW1, HMW2, and HMW3 synthesis and turnover in wild-type and class I mutant M. pneumoniae. (A) Total protein profiles at chase times of 0 h, 1 h, 4 h, and 8 h. Wild-type and mutant samples are indicated, as are the bands corresponding to HMW1, HMW2, HMW3, and P1. The asterisk corresponds to the putative P1 precursor. Protein size standards are given in kDa. (B and C) Radioimmunoprecipitation analysis of HMW1 and HMW3, respectively, after pulse–chase labeling.
