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. 1987 Jun;31(6):883–888. doi: 10.1128/aac.31.6.883

Prevalence of macrolides-lincosamides-streptogramin B resistance and erm gene classes among clinical strains of staphylococci and streptococci.

W D Jenssen, S Thakker-Varia, D T Dubin, M P Weinstein
PMCID: PMC284204  PMID: 3113328

Abstract

A total of 332 staphylococcal and 263 streptococcal isolates from three hospital microbiology laboratories were tested with erythromycin, clindamycin, and vernamycin B alpha to determine the prevalence of macrolides-lincosamides-streptogramin B resistance. Constitutive resistance was detected in 28 Staphylococcus aureus isolates (15.5%), 53 coagulase-negative staphylococci (35.1%), and 20 streptococci (7.6%). Inducible resistance was observed in 13 S. aureus isolates (7.2%), 25 coagulase-negative staphylococci (16.6%), and 2 streptococci (0.8%). Eleven coagulase-negative staphylococci (7.3%) exhibited a novel phenotype, namely inducible resistance to erythromycin and vernamycin B alpha but not clindamycin. Among the staphylococci, two variants of the inducible phenotype detected with the agar diffusion assay correlated with the presence of classical ermA or ermC genes, respectively, by dot-blot analysis. The prevalence of the staphylococcal phenotypes were different in the hospitals surveyed, and there was an apparent inverse correlation between the resistance observed and the use of erythromycin in each hospital.

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Selected References

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  1. Anthony B. F., Concepcion N. F. Group B streptococcus in a general hospital. J Infect Dis. 1975 Nov;132(5):561–567. doi: 10.1093/infdis/132.5.561. [DOI] [PubMed] [Google Scholar]
  2. Archer G. L., Dietrick D. R., Johnston J. L. Molecular epidemiology of transmissible gentamicin resistance among coagulase-negative staphylococci in a cardiac surgery unit. J Infect Dis. 1985 Feb;151(2):243–251. doi: 10.1093/infdis/151.2.243. [DOI] [PubMed] [Google Scholar]
  3. Atkinson B. A., Lorian V. Antimicrobial agent susceptibility patterns of bacteria in hospitals from 1971 to 1982. J Clin Microbiol. 1984 Oct;20(4):791–796. doi: 10.1128/jcm.20.4.791-796.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bourbeau P., Campos J. M. Current antibiotic susceptibility of group A beta-hemolytic streptococci. J Infect Dis. 1982 Jun;145(6):916–916. doi: 10.1093/infdis/145.6.916. [DOI] [PubMed] [Google Scholar]
  5. Cohen M. L., Wong E. S., Falkow S. Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak. Antimicrob Agents Chemother. 1982 Feb;21(2):210–215. doi: 10.1128/aac.21.2.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Courvalin P., Ounissi H., Arthur M. Multiplicity of macrolide-lincosamide-streptogramin antibiotic resistance determinants. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):91–100. doi: 10.1093/jac/16.suppl_a.91. [DOI] [PubMed] [Google Scholar]
  7. Dixon J. M., Lipinski A. E. Infections with beta-Hemolytic Streptococcus resistant to lincomycin and erythromycin and observations on zonal-pattern resistance to lincomycin. J Infect Dis. 1974 Oct;130(4):351–356. doi: 10.1093/infdis/130.4.351. [DOI] [PubMed] [Google Scholar]
  8. Dixon J. M., Lipinski A. E. Resistance of group A beta-hemolytic streptococci to lincomycin and erythromycin. Antimicrob Agents Chemother. 1972 Apr;1(4):333–339. doi: 10.1128/aac.1.4.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dubreuil L., Devos J., Neut C., Romond C. Susceptibility of anaerobic bacteria from several French hospitals to three major antibiotics. Antimicrob Agents Chemother. 1984 Jun;25(6):764–766. doi: 10.1128/aac.25.6.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duval J. Evolution and epidemiology of MLS resistance. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):137–149. doi: 10.1093/jac/16.suppl_a.137. [DOI] [PubMed] [Google Scholar]
  11. GARROD L. P. The erythromycin group of antibiotics. Br Med J. 1957 Jul 13;2(5036):57–63. doi: 10.1136/bmj.2.5036.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hahn J., Grandi G., Gryczan T. J., Dubnau D. Translational attenuation of ermC: a deletion analysis. Mol Gen Genet. 1982;186(2):204–216. doi: 10.1007/BF00331851. [DOI] [PubMed] [Google Scholar]
  13. Hardy K., Haefeli C. Expression in Escherichia coli of a staphylococcal gene for resistance to macrolide, lincosamide, and streptogramin type B antibiotics. J Bacteriol. 1982 Oct;152(1):524–526. doi: 10.1128/jb.152.1.524-526.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lacey R. W. Antibiotic resistance in Staphylococcus aureus and streptococci. Br Med Bull. 1984 Jan;40(1):77–83. doi: 10.1093/oxfordjournals.bmb.a071951. [DOI] [PubMed] [Google Scholar]
  15. Lacey R. W., Keyworth N., Lincoln C. Staphylococci in the U.K.: a review. J Antimicrob Chemother. 1984 Dec;14 (Suppl 500):19–25. doi: 10.1093/jac/14.suppl_d.19. [DOI] [PubMed] [Google Scholar]
  16. Lampson B. C., Parisi J. T. Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator. J Bacteriol. 1986 May;166(2):479–483. doi: 10.1128/jb.166.2.479-483.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maruyama S., Yoshioka H., Fujita K., Takimoto M., Satake Y. Sensitivity of group A streptococci to antibiotics. Prevalence of resistance to erythromycin in Japan. Am J Dis Child. 1979 Nov;133(11):1143–1145. doi: 10.1001/archpedi.1979.02130110051007. [DOI] [PubMed] [Google Scholar]
  18. Murphy E. Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus. J Bacteriol. 1985 May;162(2):633–640. doi: 10.1128/jb.162.2.633-640.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979 Jan;2(1):109–129. doi: 10.1016/0147-619x(79)90010-6. [DOI] [PubMed] [Google Scholar]
  20. Parisi J. T., Robbins J., Lampson B. C., Hecht D. W. Characterization of a macrolide, lincosamide, and streptogramin resistance plasmid in Staphylococcus epidermidis. J Bacteriol. 1981 Nov;148(2):559–564. doi: 10.1128/jb.148.2.559-564.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Price S. B., Flournoy D. J. Comparison of antimicrobial susceptibility patterns among coagulase-negative staphylococci. Antimicrob Agents Chemother. 1982 Mar;21(3):436–440. doi: 10.1128/aac.21.3.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Privitera G., Fayolle F., Sebald M. Resistance to tetracycline, erythromycin, and clindamycin in the Bacteroides fragilis group: inducible versus constitutive tetracycline resistance. Antimicrob Agents Chemother. 1981 Sep;20(3):314–320. doi: 10.1128/aac.20.3.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reig M., Campello M. G., Baquero F. Epidemiology of clindamycin resistance in the Bacteroides fragilis group. J Antimicrob Chemother. 1984 Dec;14(6):595–603. doi: 10.1093/jac/14.6.595. [DOI] [PubMed] [Google Scholar]
  24. Thakker-Varia S., Jenssen W. D., Moon-McDermott L., Weinstein M. P., Dubin D. T. Molecular epidemiology of macrolides-lincosamides-streptogramin B resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrob Agents Chemother. 1987 May;31(5):735–743. doi: 10.1128/aac.31.5.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thayer R. E. An improved method for detecting foreign DNA in plasmids of Escherichia coli. Anal Biochem. 1979 Sep 15;98(1):60–63. doi: 10.1016/0003-2697(79)90705-x. [DOI] [PubMed] [Google Scholar]
  26. Weisblum B., Demohn V. Erythromycin-inducible resistance in Staphylococcus aureus: survey of antibiotic classes involved. J Bacteriol. 1969 May;98(2):447–452. doi: 10.1128/jb.98.2.447-452.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weisblum B., Holder S. B., Halling S. M. Deoxyribonucleic acid sequence common to staphylococcal and streptococcal plasmids which specify erythromycin resistance. J Bacteriol. 1979 Jun;138(3):990–998. doi: 10.1128/jb.138.3.990-998.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weisblum B. Inducible erythromycin resistance in bacteria. Br Med Bull. 1984 Jan;40(1):47–53. doi: 10.1093/oxfordjournals.bmb.a071947. [DOI] [PubMed] [Google Scholar]
  29. Weisblum B. Inducible resistance to macrolides, lincosamides and streptogramin type B antibiotics: the resistance phenotype, its biological diversity, and structural elements that regulate expression--a review. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):63–90. doi: 10.1093/jac/16.suppl_a.63. [DOI] [PubMed] [Google Scholar]
  30. Weisblum B., Siddhikol C., Lai C. J., Demohn V. Erythromycin-inducible resistance in Staphylococcus aureus: requirements for induction. J Bacteriol. 1971 Jun;106(3):835–847. doi: 10.1128/jb.106.3.835-847.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wiedemann B., Kresken M. The incidence and development of resistance in Staphylococcus aureus from three European countries. J Antimicrob Chemother. 1984 Dec;14 (Suppl 500):27–34. doi: 10.1093/jac/14.suppl_d.27. [DOI] [PubMed] [Google Scholar]

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