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p21Cip1 was detected in both cytoplasmic and nuclear fractions of anergic T helper type 1 (Th1) cells. The Th1 cells were incubated in primary cultures with antigen ± n-butyrate. Following 6 days in primary cultures, viable cells were isolated and cell lysates were prepared. Western blot analysis of p21Cip1 and U1 SnRNP 70 was performed. This experiment has been repeated once with similar results.
