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. 1989 Jun;33(6):983–986. doi: 10.1128/aac.33.6.983

Penicillin-binding proteins of Haemophilus ducreyi.

B C Lee 1, L E Bryan 1
PMCID: PMC284271  PMID: 2788389

Abstract

The penicillin-binding protein (PBP) profile of Haemophilus ducreyi was determined by a whole-cell-labeling assay. Only two major PBPs, of molecular weights 90,000 (PBP 1) and 38,500 (PBP 2), were detected in six of eight strains studied. Competition binding experiments and the attendant morphological effects suggested that PBP 1 was either a functional amalgamation or a lack of resolution of two proteins equivalent to PBPs 1 and 3 of Escherichia coli.

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Selected References

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  1. Barbour A. G. Properties of penicillin-binding proteins in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1981 Feb;19(2):316–322. doi: 10.1128/aac.19.2.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blackmore C. A., Limpakarnjanarat K., Rigau-Pérez J. G., Albritton W. L., Greenwood J. R. An outbreak of chancroid in Orange County, California: descriptive epidemiology and disease-control measures. J Infect Dis. 1985 May;151(5):840–844. doi: 10.1093/infdis/151.5.840. [DOI] [PubMed] [Google Scholar]
  3. Brunton J., Meier M., Ehrman N., Maclean I., Slaney L., Albritton W. L. Molecular epidemiology of beta-lactamase-specifying plasmids of Haemophilus ducreyi. Antimicrob Agents Chemother. 1982 Jun;21(6):857–863. doi: 10.1128/aac.21.6.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Georgopapadakou N. H., Liu F. Y. Penicillin-binding proteins in bacteria. Antimicrob Agents Chemother. 1980 Jul;18(1):148–157. doi: 10.1128/aac.18.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hammond G. W., Slutchuk M., Scatliff J., Sherman E., Wilt J. C., Ronald A. R. Epidemiologic, clinical, laboratory, and therapeutic features of an urban outbreak of chancroid in North America. Rev Infect Dis. 1980 Nov-Dec;2(6):867–879. doi: 10.1093/clinids/2.6.867. [DOI] [PubMed] [Google Scholar]
  6. Kraus W., Höltje J. V. Two distinct transpeptidation reactions during murein synthesis in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3099–3103. doi: 10.1128/jb.169.7.3099-3103.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kreiss J. K., Koech D., Plummer F. A., Holmes K. K., Lightfoote M., Piot P., Ronald A. R., Ndinya-Achola J. O., D'Costa L. J., Roberts P. AIDS virus infection in Nairobi prostitutes. Spread of the epidemic to East Africa. N Engl J Med. 1986 Feb 13;314(7):414–418. doi: 10.1056/NEJM198602133140704. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  9. Malouin F., Bryan L. E. DNA probe technology for detection of Haemophilus influenzae. Mol Cell Probes. 1987 Sep;1(3):221–232. doi: 10.1016/0890-8508(87)90035-1. [DOI] [PubMed] [Google Scholar]
  10. Malouin F., Bryan L. E. Haemophilus influenzae penicillin-binding proteins 1a and 3 possess distinct and opposite temperature-modulated penicillin-binding activities. Antimicrob Agents Chemother. 1988 Apr;32(4):498–502. doi: 10.1128/aac.32.4.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nsanze H., Fast M. V., D'Costa L. J., Tukei P., Curran J., Ronald A. Genital ulcers in Kenya. Clinical and laboratory study. Br J Vener Dis. 1981 Dec;57(6):378–381. doi: 10.1136/sti.57.6.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parr T. R., Jr, Bryan L. E. Mechanism of resistance of an ampicillin-resistant, beta-lactamase-negative clinical isolate of Haemophilus influenzae type b to beta-lactam antibiotics. Antimicrob Agents Chemother. 1984 Jun;25(6):747–753. doi: 10.1128/aac.25.6.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pisabarro A. G., Prats R., Váquez D., Rodríguez-Tébar A. Activity of penicillin-binding protein 3 from Escherichia coli. J Bacteriol. 1986 Oct;168(1):199–206. doi: 10.1128/jb.168.1.199-206.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schmid G. P., Sanders L. L., Jr, Blount J. H., Alexander E. R. Chancroid in the United States. Reestablishment of an old disease. JAMA. 1987 Dec 11;258(22):3265–3268. [PubMed] [Google Scholar]
  15. Spratt B. G. Penicillin-binding proteins and the future of beta-lactam antibiotics. The Seventh Fleming Lecture. J Gen Microbiol. 1983 May;129(5):1247–1260. doi: 10.1099/00221287-129-5-1247. [DOI] [PubMed] [Google Scholar]
  16. Tipper D. J. Mode of action of beta-lactam antibiotics. Pharmacol Ther. 1985;27(1):1–35. doi: 10.1016/0163-7258(85)90062-2. [DOI] [PubMed] [Google Scholar]
  17. Tuomanen E. Newly made enzymes determine ongoing cell wall synthesis and the antibacterial effects of cell wall synthesis inhibitors. J Bacteriol. 1986 Aug;167(2):535–543. doi: 10.1128/jb.167.2.535-543.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waxman D. J., Strominger J. L. Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Annu Rev Biochem. 1983;52:825–869. doi: 10.1146/annurev.bi.52.070183.004141. [DOI] [PubMed] [Google Scholar]

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