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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: Virology. 2010 Jan 25;400(2):164–174. doi: 10.1016/j.virol.2009.12.019

Figure 2. Relative infectivity of chimeric viruses from representative pairs.

Figure 2

Chimeric viruses bearing patient-derived Env V1-V5 region or NL4-3 were produced by transfection of 293 T cells with the indicated proviral constructs and normalized to p24. Two ng of p24 of each virus was used to infect TZM-bl target cells in triplicates in the presence of 40 μg/ml of DEAE-dextran as described in material and methods. After 48 hrs post-infection, cells were lysed and luciferase activity was measured. The infectivity of NL 4-3 was set at 100, the infectivity of patient-derived chimeric viruses was calculated relative to this value.