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. 2010 Jan 26;59(4):907–915. doi: 10.2337/db09-1141

TABLE 1.

Mouse quantitative PCR primer sequences

Gene Mouse Q-PCR primer sequences Temperature (°C)
L32 61.2
    Forward 5′-GCCAGGAGACGACAAAAAT
    Reverse 5′-AATCCTCTTGCCCTGATCC
AgRP 61.2
    Forward 5′-TGTGTAAGGCTGCACGAGTC
    Reverse 5′-GGCAGTAGCAAAAGGCATTG
NPY 61.2
    Forward 5′-AGGCTTGAAGACCCTTCCAT
    Reverse 5′-ACAGGCAGACTGGTTTCAGG
POMC 61.2
    Forward 5′-GAGTTCAAGAGGGAGCTGGA
    Reverse 5′-GGTCATGAAGCCACCGTAAC
Lep 61.2
    Forward 5′-TTCACACACGCAGTCGGTAT
    Reverse 5′-GCTGGTGAGGACCTGTTGAT
LPL 55
    Forward 5′-TTCCAGCCAGGATGCAACA
    Reverse 5′-GGTCCACGTCTCCGAGTCC
FABP4 55.8
    Forward 5′-CATCAGCGTAAATGGGGATT
    Reverse 5′-CTTGTGGAAGTCACGCCTTT
FASN 58.7
    Forward 5′-TTGCTGGCACTACAGAATGC
    Reverse 5′-AACAGCCTCAGAGCGACAAT
ACACA 61.4
    Forward 5′-GAGAGGGGTCAAGTCCTTCC
    Reverse 5′-CTGCTGCCGTCATAAGACAA