TABLE 1.
Gene | Mouse Q-PCR primer sequences | Temperature (°C) |
---|---|---|
L32 | 61.2 | |
Forward | 5′-GCCAGGAGACGACAAAAAT | |
Reverse | 5′-AATCCTCTTGCCCTGATCC | |
AgRP | 61.2 | |
Forward | 5′-TGTGTAAGGCTGCACGAGTC | |
Reverse | 5′-GGCAGTAGCAAAAGGCATTG | |
NPY | 61.2 | |
Forward | 5′-AGGCTTGAAGACCCTTCCAT | |
Reverse | 5′-ACAGGCAGACTGGTTTCAGG | |
POMC | 61.2 | |
Forward | 5′-GAGTTCAAGAGGGAGCTGGA | |
Reverse | 5′-GGTCATGAAGCCACCGTAAC | |
Lep | 61.2 | |
Forward | 5′-TTCACACACGCAGTCGGTAT | |
Reverse | 5′-GCTGGTGAGGACCTGTTGAT | |
LPL | 55 | |
Forward | 5′-TTCCAGCCAGGATGCAACA | |
Reverse | 5′-GGTCCACGTCTCCGAGTCC | |
FABP4 | 55.8 | |
Forward | 5′-CATCAGCGTAAATGGGGATT | |
Reverse | 5′-CTTGTGGAAGTCACGCCTTT | |
FASN | 58.7 | |
Forward | 5′-TTGCTGGCACTACAGAATGC | |
Reverse | 5′-AACAGCCTCAGAGCGACAAT | |
ACACA | 61.4 | |
Forward | 5′-GAGAGGGGTCAAGTCCTTCC | |
Reverse | 5′-CTGCTGCCGTCATAAGACAA |