Figure 3.

Inhibition of the outward potassium current by glibenclamide is partially reversed by cromakalim. (A) Recordings of normalized inward and outward potassium currents in whole-cell configuration. (B) Forty minutes after perfusion with 25 μM glibenclamide, outward potassium currents were partially inhibited without any noticeable effect on inward K⁺ currents. (C) Recovering of the outward potassium current 15 min after perfusion with 100 μM cromakalim. (D) Time course of the normalized inward and outward potassium current at +80 mV. Arrows indicate current recordings illustrated in A–C. Changes of the bath solution are represented by alternating open and shaded areas. (E) Superposition of current–voltage curves of the cell in A (•), after bath perfusion (B) with 25 μM glibenclamide (▿) and finally after perfusion (C) with 100 μM cromakalim (◊). (A–E) Whole-cell capacitance was 5 pF. Seal resistance was 1.5 GΩ.