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. 1994 Sep;38(9):2207–2209. doi: 10.1128/aac.38.9.2207

Nucleotide sequence of a plasmid-mediated cephalosporinase gene (blaLAT-1) found in Klebsiella pneumoniae.

L S Tzouvelekis 1, E Tzelepi 1, A F Mentis 1
PMCID: PMC284714  PMID: 7811049

Abstract

The nucleotide sequence of the gene encoding a novel cephalosporinase (LAT-1), carried by a non-self-transferable plasmid from Klebsiella pneumoniae, has been determined. It was found that the sequence shares a high degree of homology with the Citrobacter freundii OS60 ampC structural gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauernfeind A., Chong Y., Schweighart S. Extended broad spectrum beta-lactamase in Klebsiella pneumoniae including resistance to cephamycins. Infection. 1989 Sep-Oct;17(5):316–321. doi: 10.1007/BF01650718. [DOI] [PubMed] [Google Scholar]
  2. Fosberry A. P., Payne D. J., Lawlor E. J., Hodgson J. E. Cloning and sequence analysis of blaBIL-1, a plasmid-mediated class C beta-lactamase gene in Escherichia coli BS. Antimicrob Agents Chemother. 1994 May;38(5):1182–1185. doi: 10.1128/aac.38.5.1182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Horii T., Arakawa Y., Ohta M., Ichiyama S., Wacharotayankun R., Kato N. Plasmid-mediated AmpC-type beta-lactamase isolated from Klebsiella pneumoniae confers resistance to broad-spectrum beta-lactams, including moxalactam. Antimicrob Agents Chemother. 1993 May;37(5):984–990. doi: 10.1128/aac.37.5.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lindberg F., Normark S. Sequence of the Citrobacter freundii OS60 chromosomal ampC beta-lactamase gene. Eur J Biochem. 1986 May 2;156(3):441–445. doi: 10.1111/j.1432-1033.1986.tb09601.x. [DOI] [PubMed] [Google Scholar]
  5. Lindquist S., Lindberg F., Normark S. Binding of the Citrobacter freundii AmpR regulator to a single DNA site provides both autoregulation and activation of the inducible ampC beta-lactamase gene. J Bacteriol. 1989 Jul;171(7):3746–3753. doi: 10.1128/jb.171.7.3746-3753.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Papanicolaou G. A., Medeiros A. A., Jacoby G. A. Novel plasmid-mediated beta-lactamase (MIR-1) conferring resistance to oxyimino- and alpha-methoxy beta-lactams in clinical isolates of Klebsiella pneumoniae. Antimicrob Agents Chemother. 1990 Nov;34(11):2200–2209. doi: 10.1128/aac.34.11.2200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Payne D. J., Woodford N., Amyes S. G. Characterization of the plasmid mediated beta-lactamase BIL-1. J Antimicrob Chemother. 1992 Aug;30(2):119–127. doi: 10.1093/jac/30.2.119. [DOI] [PubMed] [Google Scholar]
  8. Philippon A., Labia R., Jacoby G. Extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1989 Aug;33(8):1131–1136. doi: 10.1128/aac.33.8.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tsukamoto K., Nishida N., Tsuruoka M., Sawai T. Function of the conserved triad residues in the class C beta-lactamase from Citrobacter freundii GN346. FEBS Lett. 1990 Oct 1;271(1-2):243–246. doi: 10.1016/0014-5793(90)80416-g. [DOI] [PubMed] [Google Scholar]
  11. Tsukamoto K., Tachibana K., Yamazaki N., Ishii Y., Ujiie K., Nishida N., Sawai T. Role of lysine-67 in the active site of class C beta-lactamase from Citrobacter freundii GN346. Eur J Biochem. 1990 Feb 22;188(1):15–22. doi: 10.1111/j.1432-1033.1990.tb15365.x. [DOI] [PubMed] [Google Scholar]
  12. Tzouvelekis L. S., Tzelepi E., Mentis A. F., Tsakris A. Identification of a novel plasmid-mediated beta-lactamase with chromosomal cephalosporinase characteristics from Klebsiella pneumoniae. J Antimicrob Chemother. 1993 May;31(5):645–654. doi: 10.1093/jac/31.5.645. [DOI] [PubMed] [Google Scholar]
  13. Yamaguchi A., Adachi H., Sawai T. Identification of the active site of Citrobacter freundii beta-lactamase using dansyl-penicillin. FEBS Lett. 1987 Jun 22;218(1):126–130. doi: 10.1016/0014-5793(87)81031-1. [DOI] [PubMed] [Google Scholar]

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