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. 1994 Oct;38(10):2488–2491. doi: 10.1128/aac.38.10.2488

Detection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCR.

M Rahman 1, G Mauff 1, J Levy 1, M Couturier 1, G Pulverer 1, N Glasdorff 1, J P Butzler 1
PMCID: PMC284770  PMID: 7840594

Abstract

To study 4-quinolone resistance, the N-terminal coding region of gyrA from nalidixic acid-susceptible and -resistant isolates of Shigella dysenteriae type 1 was amplified by PCR, cloned, and sequenced. DNA sequence analysis of gyrA from nalidixic acid-resistant isolates revealed a C-to-T transition at nucleotide position 248 leading to a Ser-83-to-Leu substitution which was absent in susceptible clinical isolates. Direct HinfI digestion of PCR-amplified DNA detected similar mutations. Thus, DNA gyrase A subunit mutation Ser-83 to Leu is implicated in 4-quinolone resistance in S. dysenteriae type 1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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