Abstract
The proteinaceous component of gram-negative bacteria, which has been termed “protodyne,” enhances nonspecific host resistance while eliciting a slight pyrogenic response equivalent to 0.2% that of a typical endotoxin. Since this material still contains small amounts of carbohydrate and lipid, it was imperative to establish that its biological activities are not the result of endotoxin contamination. Evidence that the protective activity of protodyne does not result from endotoxin contamination has now been obtained by an evaluation of the Pronase digestion products of this substance. These digestion products were found to be nonpyrogenic and to contain no measurable amount of 2-keto-3-deoxyoctonate, an essential component of bacterial lipopolysaccharides.
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Selected References
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- AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BADIN J., JACKSON C., SCHUBERT M. Improved method for determination of plasma polysaccharides with tryptophan. Proc Soc Exp Biol Med. 1953 Nov;84(2):289–291. [PubMed] [Google Scholar]
- Berger F. M., Fukui G. M., Ludwig B. J., Rosselet J. P. Increase of nonspecific resistance to infection by protodyne, a protein component derived from bacterial protoplasm. Proc Soc Exp Biol Med. 1968 Feb;127(2):556–559. doi: 10.3181/00379727-127-32739. [DOI] [PubMed] [Google Scholar]
- Berger F. M. The effect of endotoxin on resistance to infection and disease. Adv Pharmacol. 1967;5:19–46. doi: 10.1016/s1054-3589(08)60653-5. [DOI] [PubMed] [Google Scholar]
- Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghalambor M. A., Levine E. M., Heath E. C. The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. 3. The isolation and characterization of 3-deoxyoctulosonic acid. J Biol Chem. 1966 Jul 10;241(13):3207–3215. [PubMed] [Google Scholar]
- Hickman J., Ashwell G. Isolation of a bacterial lipopolysaccharide from Xanthomonas campestris containing 3-acetamido-3,6-dideoxy-D-galactose and D-rhamnose. J Biol Chem. 1966 Mar 25;241(6):1424–1428. [PubMed] [Google Scholar]
- RIBI E., PERRINE T., LIST R., BROWN W., GOODE G. Use of pressure cell to prepare cell walls from Mycobacteria. Proc Soc Exp Biol Med. 1959 Mar;100(3):647–649. doi: 10.3181/00379727-100-24730. [DOI] [PubMed] [Google Scholar]
- Raff R. A., Wheat R. W. Characterization of 3-amino-3,6-dideoxy-D-glucose from a bacterial lipopolysaccharide. J Biol Chem. 1967 Oct 25;242(20):4610–4613. [PubMed] [Google Scholar]