Abstract
Synthesis of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein was determined in Saccharomyces cerevisiae during amino acid and pyrimidine starvation and during shift-up and shift-down conditions. During amino acid starvation, cell mass, cell number, and RNA continued to increase for varying periods. During amino acid and pyrimidine starvation, cell mass and RNA showed little increase, whereas total DNA increased 11 to 17%. After a shift from broth medium to a minimal defined medium, increase in RNA and protein remained at the preshift rate before assuming a lower rate. DNA increase remained at an intermediate rate during shift-down, and then dropped to a low rate. During shift-up from minimal to broth medium, increase in cell number, protein, and DNA showed varying lag periods before increasing to the new rate characteristic of broth medium; each of these quantities exhibited a step sometime in the first 2 hr after transfer to rich medium, suggesting a partial synchronous division. Immediately after shift-up, RNA synthesis assumed a high rate, and then dropped to a rate characteristic of growth in the rich medium after about 1 hr.
Full text
PDF![458](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/78ca7b9911b9/jbacter00584-0160.png)
![459](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/32101928f1bb/jbacter00584-0161.png)
![460](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/3dfca1722749/jbacter00584-0162.png)
![461](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/2b6f626da08e/jbacter00584-0163.png)
![462](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/4c06b92e944c/jbacter00584-0164.png)
![463](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/a0551527817b/jbacter00584-0165.png)
![464](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/483e1db5a555/jbacter00584-0166.png)
![465](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/fe869a986b5e/jbacter00584-0167.png)
![466](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f07d/284839/718508bc990a/jbacter00584-0168.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HANAWALT P. C., MAALOE O., CUMMINGS D. J., SCHAECHTER M. The normal DNA replication cycle. II. J Mol Biol. 1961 Apr;3:156–165. doi: 10.1016/s0022-2836(61)80042-9. [DOI] [PubMed] [Google Scholar]
- KISSANE J. M., ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed] [Google Scholar]
- KJELDGAARD N. O., MAALOE O., SCHAECHTER M. The transition between different physiological states during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):607–616. doi: 10.1099/00221287-19-3-607. [DOI] [PubMed] [Google Scholar]
- KJELDGAARD N. O. The kinetics of ribonucleic acid- and protein formation in Salmonella typhimurium during the transition between different states of balance growth. Biochim Biophys Acta. 1961 Apr 29;49:64–76. doi: 10.1016/0006-3002(61)90870-8. [DOI] [PubMed] [Google Scholar]
- Kudo Y., Imahori K. The characterization of newly synthesized RNA in step up cultures of yeast cells. J Biochem. 1965 Oct;58(4):364–372. doi: 10.1093/oxfordjournals.jbchem.a128213. [DOI] [PubMed] [Google Scholar]
- LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
- Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OGUR M., MINCKLER S., LINDEGREN G., LINDEGREN C. C. The nucleic acids in a polyploid series of Saccharomyces. Arch Biochem Biophys. 1952 Sep;40(1):175–184. doi: 10.1016/0003-9861(52)90085-4. [DOI] [PubMed] [Google Scholar]
- PIGG C. J., SORSOLI W. A., PARKS L. W. INDUCTION OF THE METHIONINE-ACTIVATING ENZYME IN SACCHAROMYCES CEREVISIAE. J Bacteriol. 1964 Apr;87:920–923. doi: 10.1128/jb.87.4.920-923.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schleif R. Control of production of ribosomal protein. J Mol Biol. 1967 Jul 14;27(1):41–55. doi: 10.1016/0022-2836(67)90350-6. [DOI] [PubMed] [Google Scholar]
- Surdin Y., Sly W., Sire J., Bordes A. M., Robichon-Szulmajster H. Propriétés et contrôle génétique du système d'accumulation des acides aminés chez Saccharomyces cerevisiae. Biochim Biophys Acta. 1965 Oct 18;107(3):546–566. [PubMed] [Google Scholar]
- Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]
- Williamson D. H. The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1965 Jun;25(3):517–528. doi: 10.1083/jcb.25.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]