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. 1969 May;98(2):511–518. doi: 10.1128/jb.98.2.511-518.1969

Morphological, Cultural, Biochemical, and Serological Comparison of Japanese Strains of Vibrio parahemolyticus with Related Cultures Isolated in the United States

Robert M Twedt 1, Procter L Spaulding 1, Herbert E Hall 1
PMCID: PMC284846  PMID: 5784207

Abstract

Morphological, cultural, biochemical, and serological characteristics of 79 strains of Vibrio parahemolyticus isolated from patients suffering from gastroenteric disease in Japan were compared with 17 suspected V. parahemolyticus cultures isolated from wound infections and 14 nonpathogenic vibrios isolated from an estuarine environment in the United States. These groups were differentiated on the basis of several key reactions which included: the range of growth temperature and salt tolerance; the production of catalase and acetoin; the hydrolysis of starch; the fermentation and utilization as single carbon source of sucrose, cellobiose, and arabinose; and the ability to swarm on 1% agar. The separation of the groups on the basis of cultural and biochemical analyses was confirmed by means of slide agglutinations with specific anti-K antisera. The results of this study strongly suggest that the wound infection isolates are V. parahemolyticus species which are easily distinguished from the nonpathogenic estuarine vibrios.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baross J., Liston J. Isolation of vibrio parahaemolyticus from the Northwest Pacific. Nature. 1968 Mar 30;217(5135):1263–1264. doi: 10.1038/2171263a0. [DOI] [PubMed] [Google Scholar]
  2. DAVIS B. R., EWING W. H. LIPOLYTIC, PECTOLYTIC, AND ALGINOLYTIC ACTIVITIES OF ENTEROBACTERIACEAE. J Bacteriol. 1964 Jul;88:16–19. doi: 10.1128/jb.88.1.16-19.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GABY W. L., HADLEY C. Practical laboratory test for the identification of Pseudomonas aeruginosa. J Bacteriol. 1957 Sep;74(3):356–358. doi: 10.1128/jb.74.3.356-358.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KAUFFMANN F., PETERSEN A. The biochemical group and type differentiation of Enterobacteriaceae by organic acids. Acta Pathol Microbiol Scand. 1956;38(6):481–491. doi: 10.1111/j.1699-0463.1956.tb04666.x. [DOI] [PubMed] [Google Scholar]
  6. Koser S. A. Utilization of the Salts of Organic Acids by the Colon-Aerogenes Group. J Bacteriol. 1923 Sep;8(5):493–520. doi: 10.1128/jb.8.5.493-520.1923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LEIFSON E. DETERMINATION OF CARBOHYDRATE METABOLISM OF MARINE BACTERIA. J Bacteriol. 1963 May;85:1183–1184. doi: 10.1128/jb.85.5.1183-1184.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEIFSON E. Staining, shape and arrangement of bacterial flagella. J Bacteriol. 1951 Oct;62(4):377–389. doi: 10.1128/jb.62.4.377-389.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leifson E. The Fermentation of Sodium Malonate as a Means of Differentiating Aerobacter and Escherichia. J Bacteriol. 1933 Sep;26(3):329–330. doi: 10.1128/jb.26.3.329-330.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
  11. SAKAZAKI R., IWANAMI S., FUKUMI H. STUDIES ON THE ENTEROPATHOGENIC, FACULTATIVELY HALOPHILIC BACTERIA, VIBRIO PARAHAEMOLYTICUS. I. MORPHOLOGICAL, CULTURAL AND BIOCHEMICAL PROPERTIES AND ITS TAXONOMICAL POSITION. Jpn J Med Sci Biol. 1963 Aug;16:161–188. doi: 10.7883/yoken1952.16.161. [DOI] [PubMed] [Google Scholar]
  12. TIDWELL W. L., HEATHER C. D., MERKLE C. An autoclave-sterilized medium for the detection of urease activity. J Bacteriol. 1955 Jun;69(6):701–702. doi: 10.1128/jb.69.6.701-702.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zen-Yoji H., Sakai S., Terayama T., Kudo Y., Ito T., Benoki M., Nagasaki M. Epidemiology, enteropathogenicity, and classification of Vi.rio parahaemolyticus. J Infect Dis. 1965 Dec;115(5):436–444. doi: 10.1093/infdis/115.5.436. [DOI] [PubMed] [Google Scholar]

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