Abstract
The secretion of H2O2 by Mycoplasma pneumoniae and M. gallisepticum was measured with the new catalase-aminotriazole method. Peroxide secretion by the mycoplasmas was stimulated by glucose. When catalase and aminotriazole were omitted and exogenous H2O2 was added to the mycoplasmas, a loss in H2O2 was noted with time; the addition of glucose speeded the disappearance of H2O2. The presence of this peroxidase-like activity in the mycoplasmas explains an observed failure of H2O2 to accumulate freely in the suspension medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHEN G., HOCHSTEIN P. GENERATION OF HYDROGEN PEROXIDE IN ERYTHROCYTES BY HEMOLYTIC AGENTS. Biochemistry. 1964 Jul;3:895–900. doi: 10.1021/bi00895a006. [DOI] [PubMed] [Google Scholar]
- COHEN G., HOCHSTEIN P. GLUTATHIONE PEROXIDASE: THE PRIMARY AGENT FOR THE ELIMINATION OF HYDROGEN PEROXIDE IN ERYTHROCYTES. Biochemistry. 1963 Nov-Dec;2:1420–1428. doi: 10.1021/bi00906a038. [DOI] [PubMed] [Google Scholar]
- Cohen G., Somerson N. L. Catalase-aminotriazole method for measuring secretion of hydrogen peroxide by microorganisms. J Bacteriol. 1969 May;98(2):543–546. doi: 10.1128/jb.98.2.543-546.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen G., Somerson N. L. Mycoplasma pneumoniae: hydrogen peroxide secretion and its possible role in virulence. Ann N Y Acad Sci. 1967 Jul 28;143(1):85–87. doi: 10.1111/j.1749-6632.1967.tb27648.x. [DOI] [PubMed] [Google Scholar]
- Dodge J. T., Cohen G., Kayden H. J., Phillips G. B. Peroxidative hemolysis of red blood cells from patients with abetalipoproteinemia (acanthocytosis). J Clin Invest. 1967 Mar;46(3):357–368. doi: 10.1172/JCI105537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karnovsky M. L. The metabolism of leukocytes. Semin Hematol. 1968 Apr;5(2):156–165. [PubMed] [Google Scholar]
- MILLS G. C. Glutathione peroxidase and the destruction of hydrogen peroxide in animal tissues. Arch Biochem Biophys. 1960 Jan;86:1–5. doi: 10.1016/0003-9861(60)90357-x. [DOI] [PubMed] [Google Scholar]
- Maisel J. C., Babbitt L. H., John T. J. Fatal Mycoplasma pneumoniae infection with isolation of organisms from lung. JAMA. 1967 Oct 23;202(4):287–290. [PubMed] [Google Scholar]
- NELSON G. H. Ethanol protection against the catalase-depressing effect of 3-amino-1,2,4-triazole. Science. 1958 Mar 7;127(3297):520–521. doi: 10.1126/science.127.3297.520. [DOI] [PubMed] [Google Scholar]
- SEELEY H. W., VANDEMARK P. J. An adaptive peroxidation by Streptococcus faecalis. J Bacteriol. 1951 Jan;61(1):27–35. doi: 10.1128/jb.61.1.27-35.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH S. L., VANDEMARK P. J., FABRICANT J. RESPIRATORY PATHWAYS IN THE MYCOPLASMA. I. LACTATE OXIDATION BY MYCOPLASMA GALLISEPTICUM. J Bacteriol. 1963 Nov;86:893–897. doi: 10.1128/jb.86.5.893-897.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Somerson N. L., James W. D., Walls B. E., Chanock R. M. Growth of Mycoplasma pneumoniae on a glass surface. Ann N Y Acad Sci. 1967 Jul 28;143(1):384–389. doi: 10.1111/j.1749-6632.1967.tb27680.x. [DOI] [PubMed] [Google Scholar]
- Somerson N. L., Walls B. E., Chanock R. M. Hemolysin of Mycoplasma pneumoniae: tentative identification as a peroxide. Science. 1965 Oct 8;150(3693):226–228. doi: 10.1126/science.150.3693.226. [DOI] [PubMed] [Google Scholar]
- Thomas L., Bitensky M. W. Methaemoglobin formation by Mycoplasma gallisepticum: the role of hydrogen peroxide. Nature. 1966 May 28;210(5039):963–964. doi: 10.1038/210963a0. [DOI] [PubMed] [Google Scholar]