Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 May;98(2):579–586. doi: 10.1128/jb.98.2.579-586.1969

Correlation Between the Rate of Ribonucleic Acid Synthesis and the Level of Valyl Transfer Ribonucleic Acid in Mutants of Escherichia coli

Sam Kaplan 1
PMCID: PMC284857  PMID: 4891259

Abstract

By use of a mutant of Escherichia coli with a partially thermolabile transfer ribonucleic acid (tRNA) synthase, it was possible to regulate the rate of RNA synthesis over a 10-fold range. The addition of chloramphenicol to cultures kept at the nonpermissive temperature stimulated RNA synthesis. The longer the culture was kept at the nonpermissive temperature prior to addition of chloramphenicol, the lower was the resulting rate of RNA synthesis. The decrease in the rate of incorporation of labeled uracil into RNA was correlated with the decrease in the level of valyl tRNA. Additional experiments provided evidence which may be interpreted as indicating that valyl tRNA does not, by itself, react with the RNA-forming system.

Full text

PDF
579

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I., SPIEGELMAN S. Protein and ribonucleic acid synthesis in a chloramphenicol-inhibited system. Biochim Biophys Acta. 1961 Oct 14;53:70–84. doi: 10.1016/0006-3002(61)90795-8. [DOI] [PubMed] [Google Scholar]
  2. EIDLIC L., NEIDHARDT F. C. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES. J Bacteriol. 1965 Mar;89:706–711. doi: 10.1128/jb.89.3.706-711.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ezekiel D. H., Valulis B. Control of ribonucleic acid synthesis in Escherichia coli cells with altered transfer ribonucleic acid concentration. Biochim Biophys Acta. 1966 Oct 24;129(1):123–139. doi: 10.1016/0005-2787(66)90014-1. [DOI] [PubMed] [Google Scholar]
  5. GROS F., GROS F. Role des aminoacides dans la synthese des acides nucléiques chez Escherichia coli. Biochim Biophys Acta. 1956 Oct;22(1):200–201. doi: 10.1016/0006-3002(56)90246-3. [DOI] [PubMed] [Google Scholar]
  6. GROS F., GROS Françoise Role des acides amines dans la synthèse des acides nucléiques chez Escherichia coli. Exp Cell Res. 1958 Feb;14(1):104–131. doi: 10.1016/0014-4827(58)90218-0. [DOI] [PubMed] [Google Scholar]
  7. KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
  8. Kaplan S., Anderson D. Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J Bacteriol. 1968 Mar;95(3):991–997. doi: 10.1128/jb.95.3.991-997.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaplan S. Chloramphenicol and the stimulation of ribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1969 May;98(2):587–592. doi: 10.1128/jb.98.2.587-592.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. MARTIN E. M., YEGIAN C., STENT G. S. SPECIFICITY OF THE AMINO ACID TRANSFER REACTION IN E. COLI STRAINS CARRYING DIFFERENT ALLELES OF THE RNA CONTROL (RC) GENE. Z Vererbungsl. 1963 Nov 21;94:303–315. doi: 10.1007/BF00894774. [DOI] [PubMed] [Google Scholar]
  12. Morris D. W., DeMoss J. A. Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):262–268. doi: 10.1073/pnas.56.1.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morris D. W., DeMoss J. A. Role of aminoacyl-transfer ribonucleic acid in the regulation of ribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1965 Dec;90(6):1624–1631. doi: 10.1128/jb.90.6.1624-1631.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nierlich D. P. Amino acid control over RNA synthesis: a re-evaluation. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345–1352. doi: 10.1073/pnas.60.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PARDEE A. B., PRESTIDGE L. S. The dependence of nucleic acid synthesis on the presence of amino acids in Escherichia coli. J Bacteriol. 1956 Jun;71(6):677–683. doi: 10.1128/jb.71.6.677-683.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES