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. 1969 May;98(2):599–604. doi: 10.1128/jb.98.2.599-604.1969

Chromosome Transfer from F-lac+ Strains of Escherichia coli K-12 Mutant at recA, recB, or recC

Brian M Wilkins 1
PMCID: PMC284860  PMID: 4891262

Abstract

The frequency of chromosome transfer from various recombination-deficient F-lac+ donor strains was estimated by standardizing the yield of conjugants receiving a male chromosomal marker against the level of episome transfer in the mating mixture. The efficiency of chromosome transfer from newly formed F-lac+ cells carrying recB21 or recC22 was more than 50% of the wild-type value, although it was about 10 and 20%, respectively, if the male cell lines had become established. In contrast, recA13 donors transmitted the chromosome with less than 10−4 of the normal frequency. If chromosome transfer from F-lac+ strains reflects the cutting and subsequent joining of homologous single strands of episomal and chromosomal deoxyribonucleic acid by recombination, these results imply that the completed unions are not made in recA cells, but can be effected with more than 50% of normal efficiency in newly formed partial diploids mutant at either recB or recC. Thus, the defective stage in recA mutants may precede strand joining, whereas the deficiency in recB or recC cells may involve a later step in recombinant formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADELBERG E. A., BURNS S. N. Genetic variation in the sex factor of Escherichia coli. J Bacteriol. 1960 Mar;79:321–330. doi: 10.1128/jb.79.3.321-330.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADELBERG E. A., PITTARD J. CHROMOSOME TRANSFER IN BACTERIAL CONJUGATION. Bacteriol Rev. 1965 Jun;29:161–172. doi: 10.1128/br.29.2.161-172.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
  5. Clowes R. C., Moody E. E. Chromosomal transfer from "recombination-deficient" strains of Escherichia coli K-12. Genetics. 1966 Apr;53(4):717–726. doi: 10.1093/genetics/53.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emmerson P. T. Recombination deficient mutants of Escherichia coli K12 that map between thy A and argA. Genetics. 1968 Sep;60(1):19–30. doi: 10.1093/genetics/60.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Franklin N. C. Extraordinary recombinational events in Escherichia coli. Their independence of the rec+ function. Genetics. 1967 Apr;55(4):699–707. doi: 10.1093/genetics/55.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ganesan A. K., Smith K. C. Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery. J Bacteriol. 1968 Aug;96(2):365–373. doi: 10.1128/jb.96.2.365-373.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gefter M. L., Becker A., Hurwitz J. The enzymatic repair of DNA. I. Formation of circular lambda-DNA. Proc Natl Acad Sci U S A. 1967 Jul;58(1):240–247. doi: 10.1073/pnas.58.1.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gellert M. Formation of covalent circles of lambda DNA by E. coli extracts. Proc Natl Acad Sci U S A. 1967 Jan;57(1):148–155. doi: 10.1073/pnas.57.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gross J. D., Caro L. G. DNA transfer in bacterial conjugation. J Mol Biol. 1966 Apr;16(2):269–284. doi: 10.1016/s0022-2836(66)80172-9. [DOI] [PubMed] [Google Scholar]
  12. Howard-Flanders P., Boyce R. P. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966;(Suppl):156+–156+. [PubMed] [Google Scholar]
  13. Howard-Flanders P., Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. doi: 10.1093/genetics/53.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
  16. PITTARD J., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. III. AN ANALYSIS OF THE RECOMBINATION EVENTS OCCURRING IN THE F' MALE AND IN THE ZYGOTES. Genetics. 1964 Jun;49:995–1007. doi: 10.1093/genetics/49.6.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PITTARD J., RAMAKRISHNAN T. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI. IV. EFFECT OF A CHROMOSOMAL DELETION ON CHROMOSOME TRANSFER. J Bacteriol. 1964 Aug;88:367–373. doi: 10.1128/jb.88.2.367-373.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rupp W. D., Ihler G. Strand selection during bacterial mating. Cold Spring Harb Symp Quant Biol. 1968;33:647–650. doi: 10.1101/sqb.1968.033.01.073. [DOI] [PubMed] [Google Scholar]
  19. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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