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. 1969 May;98(2):617–622. doi: 10.1128/jb.98.2.617-622.1969

Mixotrophic Growth of Hydrogenomonas eutropha

Sydney C Rittenberg a, Nelson S Goodman a,1
PMCID: PMC284863  PMID: 4977482

Abstract

Mixotrophic growth conditions were established by the addition of lactate to cultures of Hydrogenomonas eutropha growing autotrophically in a gaseous environment of H2, O2, and CO2 (6:2:1). The specific growth rate of mixotrophic cultures was double that of the autotrophic cultures, and lactate disappearance paralleled growth. Growth yields in mixotrophic cultures were significantly greater than those in heterotrophic cultures for equal quantities of lactate consumed. The magnitude of the increase in yield was directly proportional to the absolute growth rate at the time of lactate addition to the starting autotrophic culture and to the time under mixotrophic conditions. The specific activities of hydrogenase and ribulose diphosphate carboxylase decreased during mixotrophic growth; the total activities increased somewhat. The results suggested that the complete autotrophic and heterotrophic physiologies functioned simultaneously under mixotrophic contions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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