Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 May;98(2):623–628. doi: 10.1128/jb.98.2.623-628.1969

Biosynthesis of Branched-Chain Amino Acids in Yeast: Regulation of Synthesis of the Enzymes of Isoleucine and Valine Biosynthesis

Howard Bussey 1, H E Umbarger 1
PMCID: PMC284864  PMID: 5784215

Abstract

Regulation of the levels of the five enzymes required for the biosynthesis of isoleucine and valine was studied in a Saccharomyces sp. When a mixture of isoleucine, valine, and leucine was added to the medium, the enzymes in the wild-type strain were repressed from about 30% (transaminase B) to about 90% (acetohydroxy acid synthetase) relative to the level in minimal medium-grown cells. Repression was also observed when threonine replaced isoleucine in the mixture but not when it replaced the other two amino acids. Significant derepression relative to the level in minimal-grown cells was not obtained by growing suitably blocked auxotrophs on medium containing limiting amounts of valine, isoleucine, or leucine.

Full text

PDF
623

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dwyer S. B., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XVI. Pattern of multivalent repression in strain K-12. J Bacteriol. 1968 May;95(5):1680–1684. doi: 10.1128/jb.95.5.1680-1684.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HALVORSON H. Studies on protein and nucleic acid turnover in growing cultures of yeast. Biochim Biophys Acta. 1958 Feb;27(2):267–276. doi: 10.1016/0006-3002(58)90333-0. [DOI] [PubMed] [Google Scholar]
  4. Hartwell L. H., McLaughlin C. S. Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases. Proc Natl Acad Sci U S A. 1968 Feb;59(2):422–428. doi: 10.1073/pnas.59.2.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holzer H., Cennamo C., Boll M. Product activation of yeast threonine dehydratase by ammonia. Biochem Biophys Res Commun. 1964;14:487–492. doi: 10.1016/0006-291x(64)90256-6. [DOI] [PubMed] [Google Scholar]
  6. KAKAR S. N., WAGNER R. P. GENETIC AND BIOCHEMICAL ANALYSIS OF ISOLEUCINE-VALINE MUTANTS OF YEAST. Genetics. 1964 Feb;49:213–222. doi: 10.1093/genetics/49.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Magee P. T., Robichon-Szulmajster H. The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 2. Identification and characterization of mutants lacking the acetohydroxyacid synthetase. Eur J Biochem. 1968 Feb;3(4):502–506. doi: 10.1111/j.1432-1033.1967.tb19559.x. [DOI] [PubMed] [Google Scholar]
  8. Magee P. T., Robichon-Szulmajster H. The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 3. Properties and regulation of the activity of acetohydroxyacid synthetase. Eur J Biochem. 1968 Feb;3(4):507–511. doi: 10.1111/j.1432-1033.1967.tb19560.x. [DOI] [PubMed] [Google Scholar]
  9. Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION. J Bacteriol. 1964 Mar;87:566–573. doi: 10.1128/jb.87.3.566-573.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES. J Bacteriol. 1965 Mar;89:654–660. doi: 10.1128/jb.89.3.654-660.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robichon-Szulmajster H., Magee P. T. The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. I. Threonine deaminase. Eur J Biochem. 1968 Feb;3(4):492–501. doi: 10.1111/j.1432-1033.1967.tb19558.x. [DOI] [PubMed] [Google Scholar]
  13. STRASSMAN M., SHATTON J. B., WEINHOUSE S. Conversion of alpha-acetolactic acid to the valine precursor, alpha,beta-dihydroxyisovaleric acid. J Biol Chem. 1960 Mar;235:700–705. [PubMed] [Google Scholar]
  14. Satyanarayana T., Umbarger H. E., Lindegren G. Biosynthesis of branched-chain amino acids in yeast: regulation of leucine biosynthesis in prototrophic and leucine auxotrophic strains. J Bacteriol. 1968 Dec;96(6):2018–2024. doi: 10.1128/jb.96.6.2018-2024.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Szentirmai A., Szentirmai M., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine. J Bacteriol. 1968 May;95(5):1672–1679. doi: 10.1128/jb.95.5.1672-1679.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Szentirmai A., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XIV. Effect of thiaisoleucine. J Bacteriol. 1968 May;95(5):1666–1671. doi: 10.1128/jb.95.5.1666-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wagner R. P., Radhakrishnan A. N., Snell E. E. THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE IN NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1047–1053. doi: 10.1073/pnas.44.10.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES