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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 May;98(2):842–844. doi: 10.1128/jb.98.2.842-844.1969

Total Synthesis of Acetate from CO2 II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum

Albert Y Sun a, Lars Ljungdahl a, Harland G Wood a
PMCID: PMC284899  PMID: 5784233

Abstract

Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. Km values were: adenosine triphosphate (ATP), 6.25 × 10−4m; formate, 1.67 × 10−3m; and tetrahydrofolate, 1.00 × 10−3m; at pH 8.0. There was a requirement for divalent metals and specifically for ATP.

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Selected References

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