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. 1970 Feb;101(2):361–364. doi: 10.1128/jb.101.2.361-364.1970

Competent Diplococcus pneumoniae Accept Both Single- and Double-Stranded Deoxyribonucleic Acid

Raymond Miao 1, W R Guild 1
PMCID: PMC284915  PMID: 4391800

Abstract

The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Doty P. Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage. J Mol Biol. 1968 Mar 14;32(2):379–403. doi: 10.1016/0022-2836(68)90017-x. [DOI] [PubMed] [Google Scholar]
  2. Chevallier M. R., Bernardi G. Residual transforming activity of denatured Haemophilus influenzae DNA. J Mol Biol. 1968 Mar 14;32(2):437–451. doi: 10.1016/0022-2836(68)90020-x. [DOI] [PubMed] [Google Scholar]
  3. Chilton M. D., Hall B. D. Transforming activity in single-stranded DNA from Bacillus subtilis. J Mol Biol. 1968 Jun 28;34(3):439–451. doi: 10.1016/0022-2836(68)90171-x. [DOI] [PubMed] [Google Scholar]
  4. Chilton M. D. Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA. Science. 1967 Aug 18;157(3790):817–819. doi: 10.1126/science.157.3790.817. [DOI] [PubMed] [Google Scholar]
  5. Eigner J., Doty P. The native, denatured and renatured states of deoxyribonucleic acid. J Mol Biol. 1965 Jul;12(3):549–580. doi: 10.1016/s0022-2836(65)80312-6. [DOI] [PubMed] [Google Scholar]
  6. GUILD W. R. Evidence for intramolecular heterogeneity in pneumococcal DNA. J Mol Biol. 1963 Mar;6:214–229. doi: 10.1016/s0022-2836(63)80071-6. [DOI] [PubMed] [Google Scholar]
  7. GUILD W. R., ROBINSON M. Evidence for message reading from a unique strand of pneumococcal DNA. Proc Natl Acad Sci U S A. 1963 Jul;50:106–112. doi: 10.1073/pnas.50.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GUILD W. R. Transformation by denatured deoxyribonucleic acid. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1560–1564. doi: 10.1073/pnas.47.10.1560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guild W. R., Cato A., Jr, Lacks S. Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediate. Cold Spring Harb Symp Quant Biol. 1968;33:643–645. doi: 10.1101/sqb.1968.033.01.072. [DOI] [PubMed] [Google Scholar]
  10. LEHMAN I. R., NUSSBAUM A. L. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE). J Biol Chem. 1964 Aug;239:2628–2636. [PubMed] [Google Scholar]
  11. LERMAN L. S., TOLMACH L. J. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus. Biochim Biophys Acta. 1957 Oct;26(1):68–82. doi: 10.1016/0006-3002(57)90055-0. [DOI] [PubMed] [Google Scholar]
  12. Mulder C., Doty P. Residual activity of denatured transforming DNA of Haemophilus influenzae: a natrually occurring cross-linked DNA. J Mol Biol. 1968 Mar 14;32(2):423–435. doi: 10.1016/0022-2836(68)90019-3. [DOI] [PubMed] [Google Scholar]
  13. Peterson J. M., Guild W. R. Fractionated strands of bacterial deoxyribonucleic acid. 3. Transformation efficiencies and rates of phenotypic expression. J Bacteriol. 1968 Dec;96(6):1991–1996. doi: 10.1128/jb.96.6.1991-1996.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Porter R. D., Guild W. R. Number of transformable units per cell in Diplococcus pneumoniae. J Bacteriol. 1969 Mar;97(3):1033–1035. doi: 10.1128/jb.97.3.1033-1035.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Postel E. H., Goodgal S. H. Further studies on transformation with single-stranded DNA of Hemophilus influenzae. J Mol Biol. 1967 Sep 14;28(2):247–259. doi: 10.1016/s0022-2836(67)80007-x. [DOI] [PubMed] [Google Scholar]
  16. Postel E. H., Goodgal S. H. Uptake of "single-stranded" DNA in Hemophilus influenzae and its ability to transform. J Mol Biol. 1966 Apr;16(2):317–327. doi: 10.1016/s0022-2836(66)80175-4. [DOI] [PubMed] [Google Scholar]
  17. Rownd R., Green D. M., Sternglanz R., Doty P. Origin of the residual transforming activity of denatured Bacillus subtilis DNA. J Mol Biol. 1968 Mar 14;32(2):369–377. doi: 10.1016/0022-2836(68)90016-8. [DOI] [PubMed] [Google Scholar]
  18. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  19. VINOGRAD J., MORRIS J., DAVIDSON N., DOVE W. F., Jr The bouyant behavior of viral and bacterial DNA in alkaline CsCl. Proc Natl Acad Sci U S A. 1963 Jan 15;49:12–17. doi: 10.1073/pnas.49.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]

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