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. 1970 Feb;101(2):483–489. doi: 10.1128/jb.101.2.483-489.1970

Lipid Composition of Bacillus cereus During Growth and Sporulation

Dennis R Lang 1, D G Lundgren 1
PMCID: PMC284932  PMID: 4984075

Abstract

The lipid composition of Bacillus cereus during growth and sporulation was examined. The total lipid extract accounted for 2 to 3% of the dry weight of the cells and consisted of neutral lipids (30 to 40%) and phospholipids (60 to 70%). Phospholipids were separated by thin-layer chromatography into eight components; phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol were the major phospholipids and accounted for over 90% of the total. Also identified was a diglycosyl diglyceride and an alanine ester of phosphatidyl glycerol. Diphosphatidyl glycerol was more difficult to extract than the other components in vegetative and stationary-phase cells, but became increasingly easy to extract during spore maturation, and during sporulation cellular levels increased. Phosphatidyl glycerol had a high turnover rate; it accounted for about 70% of the phospholipid synthesis throughout sporulation but only represented between 30 and 40% of the total phospholipid at any time. Phosphatidyl ethanolamine, on the other hand, accounted for about 20% of the synthesis but was the major phospholipid (50 to 60% of the total).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertsch L. L., Bonsen P. P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XIV. Phospholipids in Bacillus megaterium. J Bacteriol. 1969 Apr;98(1):75–81. doi: 10.1128/jb.98.1.75-81.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buono F., Testa R., Lundgren D. G. Physiology of growth and sporulation in Bacillus cereus. I. Effect of glutamic and other amino acids. J Bacteriol. 1966 Jun;91(6):2291–2299. doi: 10.1128/jb.91.6.2291-2299.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Card G. L., Georgi C. E., Militzer W. E. Phospholipids from Bacillus stearothermophilus. J Bacteriol. 1969 Jan;97(1):186–192. doi: 10.1128/jb.97.1.186-192.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAWSON R. M., HEMINGTON N., DAVENPORT J. B. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses. Biochem J. 1962 Sep;84:497–501. doi: 10.1042/bj0840497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellar D. J., Lundgren D. G. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J Bacteriol. 1966 Dec;92(6):1748–1764. doi: 10.1128/jb.92.6.1748-1764.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  7. HOUTSMULLER U. M., van DEENEN L. Identification of a bacterial phospholipid as an O-ornithine ester of phosphatidyl glycerol. Biochim Biophys Acta. 1963 Apr 23;70:211–213. doi: 10.1016/0006-3002(63)90743-1. [DOI] [PubMed] [Google Scholar]
  8. Houtsmuller U. M., van Deenen L. L. On the amino acid esters of phosphatidyl glycerol from bacteria. Biochim Biophys Acta. 1965 Dec 2;106(3):564–576. doi: 10.1016/0005-2760(65)90072-x. [DOI] [PubMed] [Google Scholar]
  9. KATES M., KUSHNER D. J., JAMES A. T. The lipid composition of Bacillus cereus as influenced by the presence of alcohols in the culture medium. Can J Biochem Physiol. 1962 Jan;40:83–94. [PubMed] [Google Scholar]
  10. MATCHES J. R., WALKER H. W., AYRES J. C. PHOSPHOLIPIDS IN VEGETATIVE CELLS AND SPORES OF BACILLUS POLYMYXA. J Bacteriol. 1964 Jan;87:16–23. doi: 10.1128/jb.87.1.16-23.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Remsen C. C. The fine structure of frozen-etched Bacillus cereus spores. Arch Mikrobiol. 1966 Sep 8;54(3):266–275. doi: 10.1007/BF00408999. [DOI] [PubMed] [Google Scholar]
  12. Short S. A., White D. C., Aleem M. I. Phospholipid metabolism in Ferrobacillus ferrooxidans. J Bacteriol. 1969 Jul;99(1):142–150. doi: 10.1128/jb.99.1.142-150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  14. WINTERMANS J. F. Concentrations of phosphatides and glycolipids in leaves and chloroplasts. Biochim Biophys Acta. 1960 Oct 21;44:49–54. doi: 10.1016/0006-3002(60)91521-3. [DOI] [PubMed] [Google Scholar]
  15. den Kamp JA O. P., van Iterson W., van Deenen L. L. Studies of the phospholipids and morphology of protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1967;135(5):862–884. doi: 10.1016/0005-2736(67)90056-9. [DOI] [PubMed] [Google Scholar]
  16. den Kamp J. A., Redai I., van Deenen L. L. Phospholipid composition of Bacillus subtilis. J Bacteriol. 1969 Jul;99(1):298–303. doi: 10.1128/jb.99.1.298-303.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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