Abstract
Unlike five other strains of Mucor rouxii previously studied, certain nutritional factors must be present for rapid growth and completely yeastlike development of M. rouxii (National Regional Research Laboratory 1894) under CO2; high CO2 tensions markedly inhibit growth of this strain. Addition of yeast extract, peptone, or enzymatically hydrolyzed casein in substrate amounts to a basal medium (containing acid-hydrolyzed casein) completely relieved CO2 inhibition of growth and permitted yeastlike development. The “CO2 growth factor” activity of these supplements proved to be dialyzable and acid labile. These findings, together with the results of gel filtration and amino acid analysis, suggested that CO2 growth factor activity can be attributed to small peptides.
Full text
PDF![595](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/150e73bbe6e7/jbacter00581-0287.png)
![596](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/6945b328b9f8/jbacter00581-0288.png)
![597](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/504f81851763/jbacter00581-0289.png)
![598](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/de15304d82cd/jbacter00581-0290.png)
![599](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/e2cf59427399/jbacter00581-0291.png)
![600](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/d643d26ba66d/jbacter00581-0292.png)
![601](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/8c59b0f8e783/jbacter00581-0293.png)
![602](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ee/284946/fe9e13b47e76/jbacter00581-0294.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANDREASEN A. A., STIER T. J. B. Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. J Cell Physiol. 1953 Feb;41(1):23–36. doi: 10.1002/jcp.1030410103. [DOI] [PubMed] [Google Scholar]
- ANDREASEN A. A., STIER T. J. Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. J Cell Physiol. 1954 Jun;43(3):271–281. doi: 10.1002/jcp.1030430303. [DOI] [PubMed] [Google Scholar]
- BARTNICKI-GARCIA S., NICKERSON W. J. Induction of yeast-like development in Mucor by carbon dioxide. J Bacteriol. 1962 Oct;84:829–840. doi: 10.1128/jb.84.4.829-840.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BARTNICKI-GARCIA S., NICKERSON W. J. Nutrition, growth, and morphogenesis of Mucor rouxii. J Bacteriol. 1962 Oct;84:841–858. doi: 10.1128/jb.84.4.841-858.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bartnicki-Garcia S., Nickerson W. J. THIAMINE AND NICOTINIC ACID: ANAEROBIC GROWTH FACTORS FOR MUCOR ROUXII. J Bacteriol. 1961 Jul;82(1):142–148. doi: 10.1128/jb.82.1.142-148.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEMAIN A. L., HENDLIN D., NEWKIRK J. A. Role of fatty acids in the growth stimulation of Sarcina species by vitamin-free casein digests. J Bacteriol. 1959 Dec;78:839–843. doi: 10.1128/jb.78.6.839-843.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elmer G. W., Nickerson W. J. Filamentous growth of Mucor rouxii under nitrogen. J Bacteriol. 1970 Feb;101(2):592–594. doi: 10.1128/jb.101.2.592-594.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GUNNER H. B., ALEXANDER M. ANAEROBIC GROWTH OF FUSARIUM OXYSPORUM. J Bacteriol. 1964 Jun;87:1309–1316. doi: 10.1128/jb.87.6.1309-1316.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIHARA H., SNELL E. E. Peptides and bacterial growth. IX. Release of double inhibitions with single peptides. J Biol Chem. 1960 May;235:1415–1418. [PubMed] [Google Scholar]
- KIHARA H., SNELL E. E. Peptides and bacterial growth. VIII. The nature of strepogenin. J Biol Chem. 1960 May;235:1409–1414. [PubMed] [Google Scholar]
- LEACH F. R., SNELL E. E. Occurrence of independent uptake mechanisms for glycine and glycine peptides in Lactobacillus casei. Biochim Biophys Acta. 1959 Jul;34:292–293. doi: 10.1016/0006-3002(59)90277-x. [DOI] [PubMed] [Google Scholar]
- Richardson G. M. The nutrition of Staphylococcus aureus. Necessity for uracil in anaerobic growth. Biochem J. 1936 Dec;30(12):2184–2190. doi: 10.1042/bj0302184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stotzky G., Goos R. D. Adaptation of the soil microbiota to high CO2 and low O2 tensions. Can J Microbiol. 1966 Aug;12(4):849–861. doi: 10.1139/m66-112. [DOI] [PubMed] [Google Scholar]
- Stotzky G., Goos R. D. Effect of high CO2 and low O2 tensions on the soil microbiota. Can J Microbiol. 1965 Oct;11(5):853–868. doi: 10.1139/m65-115. [DOI] [PubMed] [Google Scholar]
- WOOLLEY D. W., MERRIFIELD R. B. Specificity of peptides; new aspects of the specificity of peptides with vitamin and hormone action are described. Science. 1958 Aug 1;128(3318):238–240. doi: 10.1126/science.128.3318.238. [DOI] [PubMed] [Google Scholar]