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. 1970 Apr;102(1):24–29. doi: 10.1128/jb.102.1.24-29.1970

Effect of Biotin on Fatty Acids and Phospholipids of Biotin-Sensitive Strains of Rhizobium japonicum1

C R Bunn a, J J McNeill a, G H Elkan a
PMCID: PMC284965  PMID: 5437727

Abstract

The effect of biotin on fatty acids and intact lipids was studied by comparing a biotin-requiring, a biotin-inhibited, and a biotin-indifferent strain of Rhizobium japonicum. These organisms were grown in a defined medium with added levels of 0, 0.3, and 0.5 μg of biotin per liter, and were analyzed for fatty acids and lipid components. Myristic, palmitic, and octadecenoic acids were found to be the major fatty acids in these strains. The indifferent strain also contained large amounts of C19 cyclopropane acid and small amounts of a C17 cyclopropane acid. Several unidentified acids were present in the other two strains. The percentages of fatty acids showed statistically significant changes corresponding with changes in level of biotin in the medium. When biotin concentration was increased in the medium, the C18 monoenoic acids of the biotin-requiring strain increased significantly, and those of the biotin-inhibited and biotin-indifferent strains decreased significantly. Palmitic acid showed a statistically significant increase in the indifferent strain with increasing biotin concentration. The principal intact lipid components in these strains are phospholipids. The major phospholipids are phosphatidylserine, phosphatidylcholine, phosphatitidylethanolamine, and cardiolipin. These phospholipids were not affected by biotin level and were independent of medium composition.

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Selected References

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  1. CROOM J. A., MCNEILL J. J., TOVE S. B. BIOTIN DEFICIENCY AND THE FATTY ACIDS OF CERTAIN BIOTIN-REQUIRING BACTERIA. J Bacteriol. 1964 Aug;88:389–394. doi: 10.1128/jb.88.2.389-394.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elkan G. H., Kwik I. Nitrogen, energy and vitamin nutrition of Rhizobium japonicum. J Appl Bacteriol. 1968 Dec;31(4):399–404. doi: 10.1111/j.1365-2672.1968.tb00386.x. [DOI] [PubMed] [Google Scholar]
  3. Elkan G. H. Some effects of medium composition and metabolic intermediates on biotin inhibition in a strain of Rhizobium japonicum. Can J Microbiol. 1967 May;13(5):533–542. doi: 10.1139/m67-068. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. GAVIN J. J., UMBREIT W. W. EFFECT OF BIOTIN ON FATTY ACID DISTRIBUTION IN ESCHERICHIA COLI. J Bacteriol. 1965 Feb;89:437–443. doi: 10.1128/jb.89.2.437-443.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOFMANN K., PANOS C. The biotin-like activity of lactobacillic acid and related compounds. J Biol Chem. 1954 Oct;210(2):687–693. [PubMed] [Google Scholar]
  7. Holden J. T., Utech N. M. Effect of biotin, pantothenic acid and nicotinic acid deficiencies on amino acid transport in Lactobacillus plantarum. Biochim Biophys Acta. 1967 Jul 3;135(3):517–531. doi: 10.1016/0005-2736(67)90041-7. [DOI] [PubMed] [Google Scholar]
  8. Hubbard J. S., Hall A. N. Effects of biotin on glutamate production and fatty acid composition in Bacillus cereus 14B22. Can J Microbiol. 1968 Oct;14(10):1039–1048. doi: 10.1139/m68-175. [DOI] [PubMed] [Google Scholar]
  9. Ikawa M. Bacterial phosphatides and natural relationships. Bacteriol Rev. 1967 Mar;31(1):54–64. doi: 10.1128/br.31.1.54-64.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaneshiro T., Thomas P. J. Methylation of fatty acids in a methionine-dependent Agrobacterium tumefaciens controlled with exogenous methionine. Biochim Biophys Acta. 1969 Jul 29;187(1):26–35. doi: 10.1016/0005-2760(69)90129-5. [DOI] [PubMed] [Google Scholar]
  11. MURPHY S. G., ELKAN G. H. GROWTH INHIBITION BY BIOTIN IN A STRAIN OF RHIZOBIUM JAPONICUM. J Bacteriol. 1963 Oct;86:884–885. doi: 10.1128/jb.86.4.884-885.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'leary W. M. THE FATTY ACIDS OF BACTERIA. Bacteriol Rev. 1962 Dec;26(4):421–447. doi: 10.1128/br.26.4.421-447.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WAKIL S. J., TITCHENER E. B., GIBSON D. M. Evidence for the participation of biotin in the enzymic synthesis of fatty acids. Biochim Biophys Acta. 1958 Jul;29(1):225–226. doi: 10.1016/0006-3002(58)90177-x. [DOI] [PubMed] [Google Scholar]

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