Abstract
The growth rate of a pyrimidine-requiring strain was controlled by limiting the concentration of exogenous orotic acid. As the steady state, pyrimidine-limited growth rate was decreased, the intracellular pyrimidine pools and the total nucleic acid per unit mass of culture also decreased. The ratio of deoxyribonucleic acid to protein remained constant, whereas the ratio of ribonucleic acid to protein decreased 30% over a threefold variation in growth rate (50- to 150-min doubling times). The intracellular uridine triphosphate and cytosine triphosphate pools also decreased (although not coordinately), and the pyrimidine biosynthetic enzymes were derepressed. Cell size was unaffected by pyrimidine-mediated variation of the growth rate.
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- BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
- Dennis P. P., Herman R. K. Control of deoxyribonucleic acid and ribonucleic acid synthesis in pyrimidine-limited Escherichia coli. J Bacteriol. 1970 Apr;102(1):124–129. doi: 10.1128/jb.102.1.124-129.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau E., Maas W. K. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli. J Bacteriol. 1968 Feb;95(2):531–539. doi: 10.1128/jb.95.2.531-539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edlin G., Stent G. S. Nucleoside triphosphate pools and the regulation of RNA synthesis in E. coli. Proc Natl Acad Sci U S A. 1969 Feb;62(2):475–482. doi: 10.1073/pnas.62.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lehman I. R., Zimmerman S. B., Adler J., Bessman M. J., Simms E. S., Kornberg A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. V. CHEMICAL COMPOSITION OF ENZYMATICALLY SYNTHESIZED DEOXYRIBONUCLEIC ACID. Proc Natl Acad Sci U S A. 1958 Dec 15;44(12):1191–1196. doi: 10.1073/pnas.44.12.1191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Long C. W., Pardee A. B. Cytidine triphosphate synthetase of Escherichia coli B. I. Purification and kinetics. J Biol Chem. 1967 Oct 25;242(20):4715–4721. [PubMed] [Google Scholar]
- Neuhard J. Pyrimidine nucleotide metabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium. J Bacteriol. 1968 Nov;96(5):1519–1527. doi: 10.1128/jb.96.5.1519-1527.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuhard J., Randerath E., Randerath K. Ion-exchange thin-layer chromatography. 13. Resolution of complex nucleoside triphosphate mixtures. Anal Biochem. 1965 Nov;13(2):211–222. doi: 10.1016/0003-2697(65)90191-0. [DOI] [PubMed] [Google Scholar]
- Nierlich D. P. Amino acid control over RNA synthesis: a re-evaluation. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345–1352. doi: 10.1073/pnas.60.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosset R., Julien J., Monier R. Ribonucleic acid composition of bacteria as a function of growth rate. J Mol Biol. 1966 Jul;18(2):308–320. doi: 10.1016/s0022-2836(66)80248-6. [DOI] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warner H. R., Barnes J. E. Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1233–1240. doi: 10.1073/pnas.56.4.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]