Abstract
Immobilized deoxyribonucleic acid (DNA) preparations from eight species of mycobacteria were reacted with labeled reference DNA from Mycobacterium tuberculosis and M. kansasii. All mycobacteria showed some degree of homology. Immobilized DNA from Pseudomonas multivorans, which has a guanine plus cytosine content within the range established for the mycobacteria, showed no significant binding with either reference system. Relative per cent binding and thermal stability of bound DNA were used as parameters for determining degrees of relatedness among members of the genus. When these relationships were compared with those established by numberical taxonomic methods, a high correlation was found.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Johnson J. L., Ordal E. J. Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. J Bacteriol. 1968 Mar;95(3):893–900. doi: 10.1128/jb.95.3.893-900.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones D., Sneath P. H. Genetic transfer and bacterial taxonomy. Bacteriol Rev. 1970 Mar;34(1):40–81. doi: 10.1128/br.34.1.40-81.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCCARTHY B. J., BOLTON E. T. An approach to the measurement of genetic relatedness among organisms. Proc Natl Acad Sci U S A. 1963 Jul;50:156–164. doi: 10.1073/pnas.50.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUNYON E. H. Anonymous mycobacteria in pulmonary disease. Med Clin North Am. 1959 Jan;43(1):273–290. doi: 10.1016/s0025-7125(16)34193-1. [DOI] [PubMed] [Google Scholar]
- Rogul M., Brendle J. J., Haapala D. K., Alexander A. D. Nucleic acid similarities among Pseudomonas pseudomallei, Pseudomonas multivorans, and Actinobacillus mallei. J Bacteriol. 1970 Mar;101(3):827–835. doi: 10.1128/jb.101.3.827-835.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tewfik E. M., Bradley S. G. Characterization of deoxyribonucleic acids from streptomycetes and nocardiae. J Bacteriol. 1967 Dec;94(6):1994–2000. doi: 10.1128/jb.94.6.1994-2000.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAYNE L. G. THE MYCOBACTERIAL MYSTIQUE: DETERRENT TO TAXONOMY. Am Rev Respir Dis. 1964 Aug;90:255–257. doi: 10.1164/arrd.1964.90.2.255. [DOI] [PubMed] [Google Scholar]
- Wayne L. G. Classification and identification of mycobacteria. 3. Species within group 3. Am Rev Respir Dis. 1966 Jun;93(6):919–928. doi: 10.1164/arrd.1966.93.6.919. [DOI] [PubMed] [Google Scholar]
- Wayne L. G., Diaz G. A. Autolysis and secondary growth of Mycobacterium tuberculosis in submerged culture. J Bacteriol. 1967 Apr;93(4):1374–1381. doi: 10.1128/jb.93.4.1374-1381.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G., Doubek J. R., Diaz G. A. Classification and indentification of mycobacteria. IV. Some important scotochromogens. Am Rev Respir Dis. 1967 Jul;96(1):88–95. doi: 10.1164/arrd.1967.96.1.88. [DOI] [PubMed] [Google Scholar]
- Wayne L. G., Gross W. M. Base composition of deoxyribonucleic acid isolated from mycobacteria. J Bacteriol. 1968 Dec;96(6):1915–1919. doi: 10.1128/jb.96.6.1915-1919.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G., Gross W. M. Isolation of deoxyribonucleic acid from mycobacteria. J Bacteriol. 1968 Apr;95(4):1481–1482. doi: 10.1128/jb.95.4.1481-1482.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G. Selection of characters for an Adansonian analysis of mycobacterial taxonomy. J Bacteriol. 1967 Apr;93(4):1382–1391. doi: 10.1128/jb.93.4.1382-1391.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]