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. 1970 Nov;104(2):635–639. doi: 10.1128/jb.104.2.635-639.1970

Iron Transport in Salmonella typhimurium: Mutants Blocked in the Biosynthesis of Enterobactin

J R Pollack 1, Bruce N Ames 1, J B Neilands 1
PMCID: PMC285038  PMID: 4923066

Abstract

A number of mutants of Salmonella typhimurium were isolated which are blocked in the biosynthesis of enterobactin, an iron chelator that is secreted by the wild-type bacteria when they are grown on low iron media. One class of these enb mutants accumulates the enterobactin precursor 2,3-dihydroxybenzoic acid, and another class does not accumulate any detectable catechol precursor. The enb mutants grow very well on a glucose-mineral salts medium. Addition of citrate, itself an iron chelator, to the medium drastically inhibits growth unless the medium is supplemented with enterobactin or iron salts. Citrate inhibits iron uptake from the medium by enb mutants; enterobactin counteracts this inhibition and also, by itself, increases iron uptake. Thus, the apparent function of enterobactin is to promote the absorption of iron from the medium by the bacteria. Transduction experiments showed that the genes for enterobactin biosynthesis are closely linked on the S. typhimurium chromosome. It is suggested that they form an operon which is repressed by the presence of iron. S. typhimurium can utilize the iron chelate ferrichrome. (Deferriferrichrome is a cyclic hexapeptide that is produced by some fungi but not by S. typhimurium.) The enb mutants use ferrichrome as an effective growth factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brot N., Goodwin J., Fales H. In vivo and in vitro formation of 2,3-dihydroxybenzoylserine by Escherichia coli K12. Biochem Biophys Res Commun. 1966 Nov 22;25(4):454–461. doi: 10.1016/0006-291x(66)90227-0. [DOI] [PubMed] [Google Scholar]
  2. Brot N., Goodwin J. Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. J Biol Chem. 1968 Feb 10;243(3):510–513. [PubMed] [Google Scholar]
  3. Corbin J. L., Bulen W. A. The isolation and identification of 2,3-dihydroxybenzoic acid and 2-N,6-N-di-92,3-dihydroxybenzoyl)-L-lysine formed by iron-deficient Azotobacter vinelandii. Biochemistry. 1969 Mar;8(3):757–762. doi: 10.1021/bi00831a002. [DOI] [PubMed] [Google Scholar]
  4. NEILANDS J. B. Factors affecting the microbial production of ferrichrome. J Biol Chem. 1953 Dec;205(2):647–650. [PubMed] [Google Scholar]
  5. O'Brien I. G., Cox G. B., Gibson F. 2,3-dihydroxy-N-benzoylserine: chemical synthesis and comparison with the natural product. Biochim Biophys Acta. 1969 Apr 1;177(2):321–328. doi: 10.1016/0304-4165(69)90142-1. [DOI] [PubMed] [Google Scholar]
  6. O'Brien I. G., Cox G. B., Gibson F. Biologically active compounds containing 2,3-dihydroxybenzoic acid and serine formed by Escherichia coli. Biochim Biophys Acta. 1970 Mar 24;201(3):453–460. doi: 10.1016/0304-4165(70)90165-0. [DOI] [PubMed] [Google Scholar]
  7. Peters W. J., Warren R. A. Phenolic acids and iron transport in Bacillus subtilis. Biochim Biophys Acta. 1968 Sep 3;165(2):225–232. doi: 10.1016/0304-4165(68)90050-0. [DOI] [PubMed] [Google Scholar]
  8. Pollack J. R., Neilands J. B. Enterobactin, an iron transport compound from Salmonella typhimurium. Biochem Biophys Res Commun. 1970 Mar 12;38(5):989–992. doi: 10.1016/0006-291x(70)90819-3. [DOI] [PubMed] [Google Scholar]
  9. ROGERS S., NEILANDS J. B. SYNTHETIC EXPERIMENTS IN THE FERRICHROME SERIES. Biochemistry. 1964 Dec;3:1850–1855. doi: 10.1021/bi00900a010. [DOI] [PubMed] [Google Scholar]
  10. SANDERSON K. E., DEMEREC M. THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM. Genetics. 1965 Jun;51:897–913. doi: 10.1093/genetics/51.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  12. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  13. Wang C. C., Newton A. Iron transport in Escherichia coli: roles of energy-dependent uptake and 2,3-dihydroxybenzoylserine. J Bacteriol. 1969 Jun;98(3):1142–1150. doi: 10.1128/jb.98.3.1142-1150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilkins T. D., Lankford C. E. Production by Salmonella typhimurium of 2,3-dihydroxybenzoylserine, and its stimulation of growth in human serum. J Infect Dis. 1970 Feb;121(2):129–136. doi: 10.1093/infdis/121.2.129. [DOI] [PubMed] [Google Scholar]
  15. Young I. G., Gibson F. Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli. Biochim Biophys Acta. 1969 May 6;177(3):401–411. doi: 10.1016/0304-4165(69)90302-x. [DOI] [PubMed] [Google Scholar]

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