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. 1970 Nov;104(2):640–645. doi: 10.1128/jb.104.2.640-645.1970

Age-Dependent Metabolic Differences in Peripheral Hyphae of Rhizoctonia solani

Boleslaw S Skowronski 1, David Gottlieb 1
PMCID: PMC285039  PMID: 5489430

Abstract

Peripheral hyphae were separated from the remaining thallus of Rhizoctonia solani in exponential and stationary phases of growth. The QO2 in whole cells of peripheral hyphae from young fungal colonies was on the average 2.6 times and the protein content 1.6 times greater than in peripheral hyphae from old fungal colonies. The overall rate of amino acid uptake was less in old than in young fungal colonies. In a polyuridylic acid-polyphenylalanine incorporating system, the two kinds of peripheral hyphae required ribosomes, supernatant fraction, polyuridylic acid, soluble ribonucleic acid, adenosine triphosphate, and pyruvate kinase. The rate of polyphenylalanine synthesis in old fungal colonies was slower than in the young fungal colonies. The ribosomes and supernatant fraction of the young and old fungal colonies were interchangeable and active. The factor responsible for deficient protein synthesis in old fungal colonies appears to be in the soluble fraction of the mycelium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bent K. J., Morton A. G. Amino acid composition of fungi during development in submerged culture. Biochem J. 1964 Aug;92(2):260–269. doi: 10.1042/bj0920260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gottlieb D., Molitoris H. P., Van Etten J. L. Changes in fungi with age. 3. Incorporation of amino acids into cells of Rhizoctonia solani and Sclerotium bataticola. Arch Mikrobiol. 1968;61(4):394–398. [PubMed] [Google Scholar]
  3. Gottlieb D., Van Etten J. L. Changes in fungi with age. Chemical composition of Rhizoctonia solani and Sclerotium bataticola. J Bacteriol. 1966 Jan;91(1):161–168. doi: 10.1128/jb.91.1.161-168.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HAYFLICK L. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. Exp Cell Res. 1965 Mar;37:614–636. doi: 10.1016/0014-4827(65)90211-9. [DOI] [PubMed] [Google Scholar]
  5. Holliday R. Errors in protein synthesis and clonal senescence in fungi. Nature. 1969 Mar 29;221(5187):1224–1228. doi: 10.1038/2211224a0. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. LUCAS J. M., SCHUURS A. H., SIMPSON M. V. A CELL-FREE AMINO ACID-INCORPORATING SYSTEM FROM SACCHAROMYCES CEREVISIAE. VARIATION IN RIBOSOMAL ACTIVITY AND IN RNA SYNTHESIS DURING LOGARITHMIC GROWTH. Biochemistry. 1964 Jul;3:959–967. doi: 10.1021/bi00895a020. [DOI] [PubMed] [Google Scholar]
  8. MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
  9. Muggleton A., Danielli J. F. Inheritance of the "life-spanning" phenomenon in Amoeba proteus. Exp Cell Res. 1968 Jan;49(1):116–120. doi: 10.1016/0014-4827(68)90524-7. [DOI] [PubMed] [Google Scholar]
  10. ORGEL L. E. The maintenance of the accuracy of protein synthesis and its relevance to ageing. Proc Natl Acad Sci U S A. 1963 Apr;49:517–521. doi: 10.1073/pnas.49.4.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rothschild H., Itikawa H., Suskind S. R. Ribosomes and ribosomal proteins from Neurospora crassa. II. Ribosomal proteins in different wild-type strains and during various stages of development. J Bacteriol. 1967 Nov;94(5):1800–1801. doi: 10.1128/jb.94.5.1800-1801.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SIEKEVITZ P. Uptake of radioactive alanine in vitro into the proteins of rat liver fractions. J Biol Chem. 1952 Apr;195(2):549–565. [PubMed] [Google Scholar]
  13. Van Etten J. L. Protein synthesis during fungal spore germination. I. Characteristics of an in vitro phenylalanine incorporating system prepared from germinated spores of Botryodiplodia theobromae. Arch Biochem Biophys. 1968 Apr;125(1):13–21. doi: 10.1016/0003-9861(68)90632-2. [DOI] [PubMed] [Google Scholar]
  14. Van Etten J., Parisi B., Ciferri O. Interchangeability of ribosomes and protein synthesizing enzymes from Penicillium cyclopium and those prepared from other organisms. Nature. 1966 Nov 26;212(5065):932–933. doi: 10.1038/212932a0. [DOI] [PubMed] [Google Scholar]

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