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. 1970 Nov;104(2):646–649. doi: 10.1128/jb.104.2.646-649.1970

Calorific Content of Certain Bacteria and Fungi

G J Prochazka 1, W J Payne 2, W R Mayberry 3
PMCID: PMC285040  PMID: 5489431

Abstract

Calorific contents of dried cells of several representative species of bacteria (gram-negative rods and gram-positive rods and cocci), two species of yeasts, and a filamentous fungus were determined by bomb calorimetry. The grand mean was 5,383 cal per g of ash-free dry weight. This value was then used to determine quantity of energy assimilated (Ea) during growth. Subsequently, Ea was employed in the equation: Ykcal = Y/(Ea + Ed), where Ykcal is the yield of cells per kilocalorie of energy taken from a culture medium, Y is the yield per mole of substrate utilized, Ea is Y times caloric content of the cells, and Ed is the energy expended by oxidative dissimilation. An estimate of Ed was obtained for a number of experiments by multiplying the moles of oxygen consumed per mole of substrate utilized during growth by the average quantity of energy utilized to reduce a mole of oxygen with electrons from organic compounds (106 kcal). From previous studies in our laboratories, a value for Ykcal of 0.118 g/kcal was predicted. The mean value for data from five studies of aerobic growth of prototrophic heterotrophs was found to be 0.111.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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