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. 2010 Jan-Mar;4(1):45–52. doi: 10.4161/pri.4.1.11074

Figure 4.

Figure 4

Time-dependent appearance of the 103Q-GFP and Sup35 polymers upon induction of 103Q-GFP synthesis. Cells of the strain 74-D694 [psi−][PIN+] with the multicopy p103Q-GFP plasmid were grown in glucose-containing medium (Gl), then in raffinose-containing medium (R), and then transferred to galactose-containing medium (Gal) and incubated for 1, 1.5, 2 and 3 h. After this, the 103Q-GFP-encoding plasmid was lost (−). (A) Polymers of 103Q-GFP and (B) Sup35 revealed by SDD-AGE. (C) The levels of polymer, monomer and total Sup35, SDS-PAGE analysis. The samples in the upper panel were not boiled before loading onto the gel. In the middle of the run, the gel was taken out, boiled to dissolve Sup35 polymers and allowed to run further. Blots were stained with anti-GFP (A) and anti-Sup35NM (B and C) antibodies.