Abstract
Membrane transport of β-alanine, l-alanine, and l-proline was studied in a β-alanine transaminaseless mutant (strain 67) of Pseudomonas fluorescens. In this mutant β-alanine is metabolically inert, and it was therefore possible to demonstrate active transport of this substrate in the absence of intracellular catabolism. The permease which catalyzes the uptake of β-alanine also transports l-proline and l-alanine. This common transport system was distinguished from permeases which transport only l-alanine and only l-proline by competition studies in strain 67 and by studies of transport specificity in a permeaseless mutant (strain 67/4MTR).
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Selected References
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- AHMED K., SCHOLEFIELD P. G. Biochemical studies on 1-aminocyclopentane carboxylic acid. Can J Biochem Physiol. 1962 Aug;40:1101–1110. [PubMed] [Google Scholar]
- AMES G. F. UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM. Arch Biochem Biophys. 1964 Jan;104:1–18. doi: 10.1016/s0003-9861(64)80028-x. [DOI] [PubMed] [Google Scholar]
- BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benko P. V., Wood T. C., Segel I. H. Multiplicity and regulation of amino acid transport in Penicillium chrysogenum. Arch Biochem Biophys. 1969 Feb;129(2):498–508. doi: 10.1016/0003-9861(69)90207-0. [DOI] [PubMed] [Google Scholar]
- CHRISTENSEN H. N. A TRANSPORT SYSTEM SERVING FOR MONO- AND DIAMINO ACIDS. Proc Natl Acad Sci U S A. 1964 Feb;51:337–344. doi: 10.1073/pnas.51.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHRISTENSEN H. N. RELATIONS IN THE TRANSPORT OF BETA-ALANINE AND THE ALPHA-AMINO ACIDS IN THE EHRLICH CELL. J Biol Chem. 1964 Oct;239:3584–3589. [PubMed] [Google Scholar]
- Gits J. J., Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. doi: 10.1016/0005-2736(67)90040-5. [DOI] [PubMed] [Google Scholar]
- Grenson M., Mousset M., Wiame J. M., Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. doi: 10.1016/0304-4165(66)90387-4. [DOI] [PubMed] [Google Scholar]
- Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):339–346. doi: 10.1016/0304-4165(66)90388-6. [DOI] [PubMed] [Google Scholar]
- Hechtman P., Scriver C. R., Middleton R. B. Isolation and properties of a beta-alanine transaminaseless mutant of Pseudomonas fluorescens. J Bacteriol. 1970 Nov;104(2):851–856. doi: 10.1128/jb.104.2.851-856.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KESSEL D., LUBIN M. Transport of proline in Escherichia coli. Biochim Biophys Acta. 1962 Feb 12;57:32–43. doi: 10.1016/0006-3002(62)91074-0. [DOI] [PubMed] [Google Scholar]
- Kay W. W., Gronlund A. F. Amino acid pool formation in Pseudomonas aeruginosa. J Bacteriol. 1969 Jan;97(1):282–291. doi: 10.1128/jb.97.1.282-291.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay W. W., Gronlund A. F. Amino acid transport in Pseudomonas aeruginosa. J Bacteriol. 1969 Jan;97(1):273–281. doi: 10.1128/jb.97.1.273-281.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay W. W., Gronlund A. F. Influence of carbon or nitrogen starvation on amino acid transport in Pseudomonas aeruginosa. J Bacteriol. 1969 Oct;100(1):276–282. doi: 10.1128/jb.100.1.276-282.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay W. W., Gronlund A. F. Isolation of amino acid transport-negative mutants of Pseudomonas aeruginosa and cells with repressed transport activity. J Bacteriol. 1969 Apr;98(1):116–123. doi: 10.1128/jb.98.1.116-123.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay W. W., Gronlund A. F. Proline transport by Pseudomonas aeruginosa. Biochim Biophys Acta. 1969;193(2):444–455. doi: 10.1016/0005-2736(69)90203-x. [DOI] [PubMed] [Google Scholar]
- Lester G. Genetic control of amino acid permeability in Neurospora crassa. J Bacteriol. 1966 Feb;91(2):677–684. doi: 10.1128/jb.91.2.677-684.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mohyuddin F., Scriver C. R. Amino acid transport in mammalian kidney: Multiple systems for imino acids and glycine in rat kidney. Am J Physiol. 1970 Jul;219(1):1–8. doi: 10.1152/ajplegacy.1970.219.1.1. [DOI] [PubMed] [Google Scholar]
- Piperno J. R., Oxender D. L. Amino acid transport systems in Escherichia coli K-12. J Biol Chem. 1968 Nov 25;243(22):5914–5920. [PubMed] [Google Scholar]
- SCHWARTZ J. H., MAAS W. K., SIMON E. J. An impaired concentrating mechanism for amino acids in mutants of Escherichia coli resistant to L-canavanine and D-serine. Biochim Biophys Acta. 1959 Apr;32:582–583. doi: 10.1016/0006-3002(59)90650-x. [DOI] [PubMed] [Google Scholar]
- SCRIVER C. R. HARTNUP DISEASE: A GENETIC MODIFICATION OF INTESTINAL AND RENAL TRANSPORT OF CERTAIN NEUTRAL ALPHA-AMINO ACIDS. N Engl J Med. 1965 Sep 2;273:530–532. doi: 10.1056/NEJM196509022731005. [DOI] [PubMed] [Google Scholar]
