Abstract
A variety of analytical techniques was employed to study the composition of the chlamydospore of Candida albicans. The outer, thin, electron-transparent layer was found to be composed of glucan, together with a small amount of chitin. The inner, thick, electron-dense layer is proteinaceous. The central structure is composed largely of ribonucleic acid and lipid globules. In addition to being acid-fast, the chlamydospore was found to contain glycolipids and to lose the property of acid-fastness on extraction with ethanol-ether.
Full text
PDF










Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burdon K. L. Fatty Material in Bacteria and Fungi Revealed by Staining Dried, Fixed Slide Preparations. J Bacteriol. 1946 Dec;52(6):665–678. doi: 10.1128/jb.52.6.665-678.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHUNG C. W., NICKERSON W. J. Polysaccharide syntheses in growing yeasts. J Biol Chem. 1954 May;208(1):395–407. [PubMed] [Google Scholar]
- DEINEMA M. H., LANDHEER C. A. Extracellular lipid production by a strain of Rhodotorula graminis. Biochim Biophys Acta. 1960 Jan 1;37:178–179. doi: 10.1016/0006-3002(60)90106-2. [DOI] [PubMed] [Google Scholar]
- Jansons V. K., Nickerson W. J. Induction, morphogenesis, and germination of the chlamydospore of Candida albicans. J Bacteriol. 1970 Nov;104(2):910–921. doi: 10.1128/jb.104.2.910-921.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEEBLE S. A., JAY R. F. Fluorescent staining for the differentiation of intracellular ribonucleic acid and deoxyribonucleic acid. Nature. 1962 Feb 17;193:695–696. doi: 10.1038/193695a0. [DOI] [PubMed] [Google Scholar]
- KOBAYASHI G. S., FRIEDMAN L., KOFROTH J. F. SOME CYTOLOGICAL AND PATHOGENIC PROPERTIES OF SPHEROPLASTS OF CANDIDA ALBICANS. J Bacteriol. 1964 Sep;88:795–801. doi: 10.1128/jb.88.3.795-801.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACLENNAN A. P., RANDALL H. M., SMITH D. W. The occurence of methyl ethers of rhamose and fucose in specific glycolipids of certain mycobacteria. Biochem J. 1961 Aug;80:309–318. doi: 10.1042/bj0800309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MERDINGER E., DEVINE E. M., Jr LIPIDS OF DEBARYOMYCES HANSENII. J Bacteriol. 1965 Jun;89:1488–1493. doi: 10.1128/jb.89.6.1488-1493.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NICKERSON W. J., DURAND S. C. KERATINASE. II. PROPERTIES OF THE CRYSTALLINE ENZYME. Biochim Biophys Acta. 1963 Sep 3;77:87–99. doi: 10.1016/0006-3002(63)90471-2. [DOI] [PubMed] [Google Scholar]
- ROBINOW C. F. Mitosis in the yeast Lipomyces lipofer. J Biophys Biochem Cytol. 1961 Apr;9:879–892. doi: 10.1083/jcb.9.4.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SVIHLA G., SCHLENK F., DAINKO J. L. Spheroplasts of the yeast Candida utilis. J Bacteriol. 1961 Dec;82:808–814. doi: 10.1128/jb.82.6.808-814.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skujins J. J., Potgieter H. J., Alexander M. Dissolution of fungal cell walls by a streptomycete chitinase and beta-(1-3) glucanase. Arch Biochem Biophys. 1965 Aug;111(2):358–364. doi: 10.1016/0003-9861(65)90197-9. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
- WALDRON D. M. Sugar components of blood and urinary glycoproteins: confirmation of the presence of fucose by a new reaction. Nature. 1952 Sep 13;170(4324):461–462. doi: 10.1038/170461b0. [DOI] [PubMed] [Google Scholar]
- Weyman-Rzucidlo D. Histochemical and immunological study of Candida albicans chlamydospores. I. Histochemical properties of C. albicans chlamydospores (Double staining method of chlamydospores). Acta Microbiol Pol. 1965;14(3):237–245. [PubMed] [Google Scholar]