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. 1970 Nov;104(2):940–947. doi: 10.1128/jb.104.2.940-947.1970

Gliding Motility Mutants of Myxococcus xanthus

Robert P Burchard 1
PMCID: PMC285078  PMID: 4923078

Abstract

Two gliding motility mutants of Myxococcus xanthus are described. The semimotile mutant (SM) originated by high-frequency segregation from the motile FBt strain. Segregation was enhanced by acridine dye treatment. SM cells glide only when apposed to other cells in a swarm. The nonmotile strain (NM) originated by mutation from SM. NM cells neither glide individually nor cooperatively. FBt, SM, and NM are indistinguishable with respect to fine structure, vegetative growth rate, glycerol-induced microcyst formation, spheroplasting, bacteriophage sensitivity, and responses to light. The motility mutants are more resistant to penicillin and more sensitive to actinomycin D than is the gliding wild type. The NM mutant is also a morphogenetic mutant; it is unable to form fruiting bodies.

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Selected References

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  1. Burchard R. P., Dworkin M. A bacteriophage for Myxococcus xanthus: isolation, characterization and relation of infectivity to host morphogenesis. J Bacteriol. 1966 Mar;91(3):1305–1313. doi: 10.1128/jb.91.3.1305-1313.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burchard R. P., Dworkin M. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J Bacteriol. 1966 Feb;91(2):535–545. doi: 10.1128/jb.91.2.535-545.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DWORKIN M., GIBSON S. M. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. doi: 10.1126/science.146.3641.243. [DOI] [PubMed] [Google Scholar]
  4. DWORKIN M. NUTRITIONAL REGU.ATION OF MORPHOGENESIS IN MYXOCOCCUS XANTHUS. J Bacteriol. 1963 Jul;86:67–72. doi: 10.1128/jb.86.1.67-72.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doetsch R. N., Hageage G. J. Motility in procaryotic organisms: problems, points of view, and perspectives. Biol Rev Camb Philos Soc. 1968 Aug;43(3):317–362. doi: 10.1111/j.1469-185x.1968.tb00963.x. [DOI] [PubMed] [Google Scholar]
  6. Dworkin M. Biology of the myxobacteria. Annu Rev Microbiol. 1966;20:75–106. doi: 10.1146/annurev.mi.20.100166.000451. [DOI] [PubMed] [Google Scholar]
  7. Dworkin M. Sensitivity of gliding bacteria to actinomycin D. J Bacteriol. 1969 May;98(2):851–852. doi: 10.1128/jb.98.2.851-852.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gräf W. Bewegungsorganellen bei Myxobakterien. Arch Hyg Bakteriol. 1965 Jul;149(5):518–526. [PubMed] [Google Scholar]
  9. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holt S. C., Leadbetter E. R. Fine structure of Sporocytophaga myxococcoides. Arch Mikrobiol. 1967 Jun 21;57(3):199–213. doi: 10.1007/BF00405947. [DOI] [PubMed] [Google Scholar]
  11. Jahn T. L., Bovee E. C. Movement and locomotion of microorganisms. Annu Rev Microbiol. 1965;19:21–58. doi: 10.1146/annurev.mi.19.100165.000321. [DOI] [PubMed] [Google Scholar]
  12. MASON D. J., POWELSON D. Lysis of Myxococcus xanthus. J Gen Microbiol. 1958 Aug;19(1):65–70. doi: 10.1099/00221287-19-1-65. [DOI] [PubMed] [Google Scholar]
  13. Pate J. L., Ordal E. J. The fine structure of Chondrococcus columnaris. 3. The surface layers of Chondrococcus columnaris. J Cell Biol. 1967 Oct;35(1):37–51. doi: 10.1083/jcb.35.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
  15. Reichenbach H. Die wahre Natur der Myxobakterien- "Rhapidosomen". Arch Mikrobiol. 1967 Apr 17;56(4):371–383. [PubMed] [Google Scholar]
  16. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. J Bacteriol. 1961 May;81:679–683. doi: 10.1128/jb.81.5.679-683.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. White D., Dworkin M., Tipper D. J. Peptidoglycan of Myxococcus xanthus: structure and relation to morphogenesis. J Bacteriol. 1968 Jun;95(6):2186–2197. doi: 10.1128/jb.95.6.2186-2197.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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