Skip to main content
. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2007 Jan 16;46(2):534–542. doi: 10.1021/bi061859h

FIGURE 6.

FIGURE 6

Comparison of tyrosyl radical, oxyferryl heme, and peroxidase inactivation kinetics of Fe-PGHS-1 during reaction with EtOOH. Fe-PGHS-1 (30 µM) in 100 mM KPi (pH 7.2), 0.04% octyl glucoside, and 10% glycerol was reacted with 20 equiv of EtOOH at room temperature. (A) Time courses of residual peroxidase activity (♦; fit,─), overall tyrosyl radical intensity (○; fit, ─ ─ ─), deconvoluted WD tyrosyl radical intensity (▲; fit, •••), and oxyferryl heme (─••─). (B) EPR spectra from reaction samples freeze-trapped at the indicated times. EPR conditions: microwave power, 1 mW; frequency, 9.297 GHz; modulation amplitude, 2 G; and temperature, 114 K.