Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 May;106(2):479–486. doi: 10.1128/jb.106.2.479-486.1971

Kinetic Characteristics of the Two Glucose Transport Systems in Neurospora crassa

R P Schneider a,1, W R Wiley a
PMCID: PMC285119  PMID: 5573732

Abstract

Glucose is transported across the cell membrane of Neurospora crassa by two physiologically and kinetically distinct transport systems. System II is repressed by growth of the cells in 0.1 m glucose. System I is synthesized constitutively. The apparent Km for glucose uptake by system I and system II are 25 and 0.04 mm, respectively. Both uptake systems are temperature dependent, and are inhibited by NaN3 and 2,4-dinitrophenol. Glucose uptake by system II was not inhibited by fructose, galactose, or lactose. However, glucose was shown to be a noncompetitive inhibitor of fructose and galactose uptake. The transport rate of [14C]3-0-methyl-d-glucose (3-0-MG) was higher in cells preloaded with unlabeled 3-0-MG than in control cells. The rate of entry of labeled 3-0-MG was only slightly inhibited by the presence of NaN3 in the medium. Further, NaN3 caused a rapid efflux of accumulated [14C]3-0-MG. These data imply that the energetic step in the transport process prevents efflux.

Full text

PDF
479

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown C. E., Romano A. H. Evidence against necessary phosphorylation during hexose transport in Aspergillus nidulans. J Bacteriol. 1969 Dec;100(3):1198–1203. doi: 10.1128/jb.100.3.1198-1203.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Crocken B., Tatum E. L. Sorbose transport in Neurospora crassa. Biochim Biophys Acta. 1967 Feb 1;135(1):100–105. doi: 10.1016/0005-2736(67)90011-9. [DOI] [PubMed] [Google Scholar]
  3. DeBusk B. G., DeBusk A. G. Molecular transport in Neurospora crassa. I. Biochemical properties of a phenylalanine permease. Biochim Biophys Acta. 1965 Jun 15;104(1):139–150. doi: 10.1016/0304-4165(65)90229-1. [DOI] [PubMed] [Google Scholar]
  4. EGAN J. B., MORSE M. L. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT. Biochim Biophys Acta. 1965 Feb 15;97:310–319. doi: 10.1016/0304-4165(65)90096-6. [DOI] [PubMed] [Google Scholar]
  5. Fox C. F., Kennedy E. P. Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli. Proc Natl Acad Sci U S A. 1965 Sep;54(3):891–899. doi: 10.1073/pnas.54.3.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOFFEE P., ENGLESBERG E., LAMY F. THE GLUCOSE PERMEASE SYSTEM IN BACTERIA. Biochim Biophys Acta. 1964 Mar 30;79:337–350. [PubMed] [Google Scholar]
  7. KOCH A. L. THE ROLE OF PERMEASE IN TRANSPORT. Biochim Biophys Acta. 1964 Jan 27;79:177–200. doi: 10.1016/0926-6577(64)90050-6. [DOI] [PubMed] [Google Scholar]
  8. Kashket E. R., Wilson T. H. Isolation and properties of mutants of Escherichia coli with increased phosphorylations of thiomethyl-beta-galactoside. Biochim Biophys Acta. 1969;193(2):294–307. doi: 10.1016/0005-2736(69)90190-4. [DOI] [PubMed] [Google Scholar]
  9. LESTER G., AZZENA D., HECHTER O. Permeability and metabolism of lactose in Neurospora crassa. J Bacteriol. 1962 Aug;84:217–227. doi: 10.1128/jb.84.2.217-227.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OKADA H., HALVORSON H. O. UPTAKE OF ALPHA-THIOETHYL D-GLUCOPYRANOSIDE BY SACCHAROMYCES CEREVISIAE. II. GENERAL CHARACTERISTICS OF AN ACTIVE TRANSPORT SYSTEM. Biochim Biophys Acta. 1964 Mar 16;82:547–555. doi: 10.1016/0304-4165(64)90446-5. [DOI] [PubMed] [Google Scholar]
  11. Robbie J. P., Wilson T. H. Transmembrane effects of beta-galactosides on thiomethyl-beta-galactoside transport in Escherichia coli. Biochim Biophys Acta. 1969 Mar 11;173(2):234–244. doi: 10.1016/0005-2736(69)90107-2. [DOI] [PubMed] [Google Scholar]
  12. SLAYMAN C. W., TATUM E. L. POTASSIUM TRANSPORT IN NEUROSPORA. I. INTRACELLULAR SODIUM AND POTASSIUM CONCENTRATIONS, AND CATION REQUIREMENTS FOR GROWTH. Biochim Biophys Acta. 1964 Nov 29;88:578–592. [PubMed] [Google Scholar]
  13. Scarborough G. A. Sugar transport in Neurospora crassa. II. A second glucose transport system. J Biol Chem. 1970 Aug 10;245(15):3985–3987. [PubMed] [Google Scholar]
  14. Scarborough G. A. Sugar transport in Neurospora crassa. J Biol Chem. 1970 Apr 10;245(7):1694–1698. [PubMed] [Google Scholar]
  15. Schachter D., Mindlin A. J. Dual influx model of thiogalactoside accumulation in Escherichia coli. J Biol Chem. 1969 Apr 10;244(7):1808–1816. [PubMed] [Google Scholar]
  16. Schneider R. P., Wiley W. R. Regulation of sugar transport in Neurospora crassa. J Bacteriol. 1971 May;106(2):487–492. doi: 10.1128/jb.106.2.487-492.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wiley W. R., Matchett W. H. Tryptophan transport in Neurospora crassa. I. Specificity and kinetics. J Bacteriol. 1966 Dec;92(6):1698–1705. doi: 10.1128/jb.92.6.1698-1705.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wong P. T., Wilson T. H. Counterflow of galactosides in Escherichia coli. Biochim Biophys Acta. 1970;196(2):336–350. doi: 10.1016/0005-2736(70)90021-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES