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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2006 Jan 17;45(2):523–532. doi: 10.1021/bi051235w

Figure 7.

Figure 7

Kinetics of cyclooxygenase inhibition by nimesulide in PGHS-2 and the Y504F and Y348F/Y504F mutants. The initial velocity of oxygen consumption was measured after preincubation of PGHS-2 (A), the Y504F mutant (B), or the Y348F/Y504F double mutant (C) with the indicated levels of nimesulide for the indicated times. Data are the averages of triplicate measurements, normalized to the control; standard deviations are given by error bars. The curves represent theoretical fits to the data using the model described by Callan et al. (30).