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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971

Beginning a Genetic Analysis of Conjugational Transfer Determined by the F Factor in Escherichia coli by Isolation and Characterization of Transfer-Deficient Mutants

Mark Achtman 1,2, Neil Willetts 1,2, Alvin J Clark 1,2
PMCID: PMC285127  PMID: 4929865

Abstract

Eighty-four transfer-deficient mutants of Flac have been isolated; 27 of these bear amber mutations and 1 mutant is temperature-sensitive. All the mutants transfer between 10−2 and <10−5% as well as wild-type Flac, all are curable by acridine orange treatment, and all are resistant to the female-specific phage φII. Some of the mutants are partially sensitive to female-specific phage tau. Sixty-three of the mutants are resistant to the male-specific phages f1, f2, and Qβ; 15 are resistant only to f2; and 6 are sensitive to all three male-specific phages. Most of the mutants are still poor recipients in conjugation, but four of the mutants resistant to f1, f2, and Qβ have become good recipients. Those mutants resistant to all three male-specific phages do not seem to make F-pili.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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