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. 1971 May;106(2):578–587. doi: 10.1128/jb.106.2.578-587.1971

Purification and Properties of l-Asparaginase from Serratia marcescens1

John W Boyd a,2, Arthur W Phillips a
PMCID: PMC285133  PMID: 4995652

Abstract

The purification and properties of a tumor inhibitory l-asparaginase from Serratia marcescens are described. The following properties of the enzyme were examined: kinetics of the enzyme reaction, catalytic activity as a function of pH, boundary sedimentation velocity, electrophoresis on polyacrylamide gel, immuno-electrophoresis against homologous and heterologous antisera, immunodiffusion, blood clearance rate in mice, and inhibition of the 6C3HED lymphoma in C3H mice. Complete regression of this tumor was obtained with a smaller dose of the enzyme from S. marcescens than with enzyme from Escherichia coli. The reason for this difference was not evident from a comparison of several properties of the two enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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