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. 1971 May;106(2):596–602. doi: 10.1128/jb.106.2.596-602.1971

Variations in the Localization of Acetyl-Coenzyme A Synthetase in Aerobic Yeast Cells

Harold P Klein 1, Linda Jahnke 1
PMCID: PMC285135  PMID: 4102333

Abstract

In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARRIGONI O., SINGER T. P. Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases. Nature. 1962 Mar 31;193:1256–1258. doi: 10.1038/1931256a0. [DOI] [PubMed] [Google Scholar]
  2. Aas M., Bremer J. Short-chain fatty acid activation in rat liver. A new assay procedure for the enzymes and studies on their intracellular localization. Biochim Biophys Acta. 1968 Oct 22;164(2):157–166. doi: 10.1016/0005-2760(68)90142-2. [DOI] [PubMed] [Google Scholar]
  3. Avers C. J., Federman M. The occurrence in yeast of cytoplasmic granules which resemble microbodies. J Cell Biol. 1968 May;37(2):555–559. doi: 10.1083/jcb.37.2.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Castelli A., Parenti-Castelli G., Bertoli E., Lenaz G. Studies on the morphogenesis of yeast mitochondria. 1. Development of mitochondrial functions during the growth phases of Saccharomyces cerevisiae. Ital J Biochem. 1969 Jan-Feb;18(1):35–59. [PubMed] [Google Scholar]
  5. Duntze W., Neumann D., Gancedo J. M., Atzpodien W., Holzer H. Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. Eur J Biochem. 1969 Aug;10(1):83–89. doi: 10.1111/j.1432-1033.1969.tb00658.x. [DOI] [PubMed] [Google Scholar]
  6. Henson C. P., Perlman P., Weber C. N., Mahler H. R. Formation of yeast mitochondria. II. Effects of antibiotics on enzyme activity during derepression. Biochemistry. 1968 Dec;7(12):4445–4454. doi: 10.1021/bi00852a041. [DOI] [PubMed] [Google Scholar]
  7. KLEIN H. P. Some observations on a cell free lipid synthesizing system from Saccharomyces cerevisiae. J Bacteriol. 1957 Apr;73(4):530–543. doi: 10.1128/jb.73.4.530-537.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein H. P., Jahnke L. Cellular localization of acetyl-coenzyme A synthetase in yeast. J Bacteriol. 1968 Nov;96(5):1632–1639. doi: 10.1128/jb.96.5.1632-1639.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein H. P., Volkmann C., Weibel J. Membranes of Saccharomyces cerevisiae. J Bacteriol. 1967 Aug;94(2):475–481. doi: 10.1128/jb.94.2.475-481.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levy M. R. Localization of acetyl-coenzyme A synthetase on peroxisomes in Tetrahymena. Biochem Biophys Res Commun. 1970 Apr 8;39(1):1–6. doi: 10.1016/0006-291x(70)90748-5. [DOI] [PubMed] [Google Scholar]
  11. Lukins H. B., Tham S. H., Wallace P. G., Linnane A. W. Correlation of membrane bound succinate dehydrogenase with the occurrence of mitochondrial profiles in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1966 May 25;23(4):363–367. doi: 10.1016/0006-291x(66)90734-0. [DOI] [PubMed] [Google Scholar]
  12. MERKENSCHLAGER M., SCHLOSSMANN K., KURZ W. Ein mechanischer Zellhomogenisator und seine Anwendbarkeit auf biologische Probleme. Biochem Z. 1957;329(4):332–340. [PubMed] [Google Scholar]
  13. MILLERD A., BONNER J. Acetate activation and acetoacetate formation in plant systems. Arch Biochem Biophys. 1954 Apr;49(2):343–355. doi: 10.1016/0003-9861(54)90204-0. [DOI] [PubMed] [Google Scholar]
  14. Matile P., Bahr G. F. Biochemical and quantitative electron microscopic evidence for heterogeneity of mitochondria from Saccharomyces cerevisiae. Exp Cell Res. 1968 Oct;52(2):301–307. doi: 10.1016/0014-4827(68)90471-0. [DOI] [PubMed] [Google Scholar]
  15. Neidle A., van den Berg C. J., Grynbaum A. The heterogeneity of rat brain mitochondria isolated on continuous sucrose gradients. J Neurochem. 1969 Feb;16(2):225–234. doi: 10.1111/j.1471-4159.1969.tb05940.x. [DOI] [PubMed] [Google Scholar]
  16. Nurminen T., Suomalainen H. Location and activity of the respiratory enzymes of baker's yeast and brewer's bottom yeast grown under anaerobic and aerobic conditions. J Gen Microbiol. 1968 Sep;53(2):275–285. doi: 10.1099/00221287-53-2-275. [DOI] [PubMed] [Google Scholar]
  17. Perlman P. S., Mahler H. R. Intracellular localization of enzymes in yeast. Arch Biochem Biophys. 1970 Jan;136(1):245–259. doi: 10.1016/0003-9861(70)90348-6. [DOI] [PubMed] [Google Scholar]
  18. Webster L. T., Jr Studies of the acetyl coenzyme A synthetase reaction. IV. The requirement for monovalent cations. J Biol Chem. 1966 Dec 10;241(23):5504–5510. [PubMed] [Google Scholar]

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