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. 1971 May;106(2):626–633. doi: 10.1128/jb.106.2.626-633.1971

Membrane Association of Conjugally Transferred Deoxyribonucleic Acid in Escherichia coli Minicells

Fred W Shull Jr 1,2,3, Joe A Fralick 1,2,3,1, Lewis P Stratton 1,2,3, William D Fisher 1,2,3
PMCID: PMC285139  PMID: 4929870

Abstract

The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells (“minicells”) of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under conditions which reduce the DNA to one-sixth its original size and suggests the possibility of multiple DNA-membrane association sites. Preliminary enzymatic studies indicate the involvement of protein, DNA, and lipids in the membrane association of the DNA.

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Selected References

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