(A) Optical trapping record of forced-backward movement of Dyn1471kDa under 10 pN super-stall force (force-feedback mode, gray-shaded area) following a motor run under increasing rearward load (nonfeedback, upper corner left, the red coordinate system indicates the corresponding force) in the presence of 1 mM ATP (trap stiffness: k = 0.056 pN/nm).
(B) Forward movement of Dyn1471kDa under a –3 pN assisting load and 1 mM ATP (trap stiffness: k = 0.033 pN/nm).
(C) Velocity (absolute values) of Dyn1471kDa movement at 10 pN rearward load (n = 54–77) and –3 pN forward load (n = 52–60), respectively, as a function of ATP concentration. Values are displayed as mean ± SEM.