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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Cell. 2007 Nov 30;131(5):952–965. doi: 10.1016/j.cell.2007.10.016

Figure 2. Forced-Backward and Assisted-Forward Movement of Full-Length Dynein in the Presence of ATP Hydrolysis.

Figure 2

(A) Optical trapping record of forced-backward movement of Dyn1471kDa under 10 pN super-stall force (force-feedback mode, gray-shaded area) following a motor run under increasing rearward load (nonfeedback, upper corner left, the red coordinate system indicates the corresponding force) in the presence of 1 mM ATP (trap stiffness: k = 0.056 pN/nm).

(B) Forward movement of Dyn1471kDa under a –3 pN assisting load and 1 mM ATP (trap stiffness: k = 0.033 pN/nm).

(C) Velocity (absolute values) of Dyn1471kDa movement at 10 pN rearward load (n = 54–77) and –3 pN forward load (n = 52–60), respectively, as a function of ATP concentration. Values are displayed as mean ± SEM.