Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Feb;113(2):711–717. doi: 10.1128/jb.113.2.711-717.1973

Protein Synthesis and Deoxyribonucleic Acid-Membrane Attachment During Thymineless Death in Escherichia coli

Frances Dankberg 1, Donald J Cummings 1
PMCID: PMC285285  PMID: 4570604

Abstract

The proteins synthesized during thymineless death in Escherichia coli B and B/r were analyzed by polyacrylamide gel elctrophoresis. It was found that the amount of a protein of molecular weight 80,000 to 88,000 is greatly increased during thymineless death compared to the amounts of other cell proteins. A technique for the isolation of cell membrane-deoxyribonucleic acid (DNA)-nascent ribonucleic acid (RNA) complex on detergent crystals was used to determine whether DNA might be detached from the cell membrane as a result of thymineless death. It was found that under no conditions of thymineless death or immunity to thymineless death was there any change in the attachment of DNA or pulse-labeled RNA to cell membrane.

Full text

PDF
711

Images in this article

713Image
on p.713

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNER H. D., COHEN S. S. The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. J Bacteriol. 1954 Jul;68(1):80–88. doi: 10.1128/jb.68.1.80-88.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Biswas C., Hardy J., Beck W. S. Release of repressor control of ribonucleotide reductase by thymine starvation. J Biol Chem. 1965 Sep;240(9):3631–3640. [PubMed] [Google Scholar]
  3. Brown N. C., Canellakis Z. N., Lundin B., Reichard P., Thelander L. Ribonucleoside diphosphate reductase. Purification of the two subunits, proteins B1 and B2. Eur J Biochem. 1969 Jul;9(4):561–573. doi: 10.1111/j.1432-1033.1969.tb00646.x. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. S., Barner H. D. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1954 Oct;40(10):885–893. doi: 10.1073/pnas.40.10.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cummings D. J., Kusy A. R. Thymineless death in Escherichia coli: deoxyribonucleic acid replication and the immune state. J Bacteriol. 1970 Apr;102(1):106–117. doi: 10.1128/jb.102.1.106-117.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cummings D. J., Kusy A. R. Thymineless death in Escherichia coli: inactivation and recovery. J Bacteriol. 1969 Aug;99(2):558–566. doi: 10.1128/jb.99.2.558-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cummings D. J., Mondale L. Thymineless death in Escherichia coli: strain specificity. J Bacteriol. 1967 Jun;93(6):1917–1924. doi: 10.1128/jb.93.6.1917-1924.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cummings D. J., Taylor A. L. Thymineless death and its relation to UV sensitivity in Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):171–176. doi: 10.1073/pnas.56.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Deutsch C. E., Pauling C. Survival and macromolecular synthesis during incubation of Escherichia coli in limiting thymine. J Bacteriol. 1971 Apr;106(1):197–203. doi: 10.1128/jb.106.1.197-203.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREIFELDER D., MAALOE O. ENERGY REQUIREMENT FOR THYMINELESS DEATH IN CELLS OF ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:987–990. doi: 10.1128/jb.88.4.987-990.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inouye M., Pardee A. B. Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli. J Biol Chem. 1970 Nov 10;245(21):5813–5819. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  14. Masker W. E., Eberle H. DNA replication and proteins that bind to DNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2549–2553. doi: 10.1073/pnas.68.10.2549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PRITCHARD R. H., LARK K. G. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:288–307. doi: 10.1016/s0022-2836(64)80208-4. [DOI] [PubMed] [Google Scholar]
  16. Reichenbach D. L., Schaiberger G. E., Sallman B. The effect of thymine starvation on chromosomal structure of Escherichia coli JG-151. Biochem Biophys Res Commun. 1971 Jan 8;42(1):23–30. doi: 10.1016/0006-291x(71)90356-1. [DOI] [PubMed] [Google Scholar]
  17. Reiter H., Ramareddy G. Loss of DNA behind the growing point of thymine-starved Bacillus subtilis 168. J Mol Biol. 1970 Jun 14;50(2):533–548. doi: 10.1016/0022-2836(70)90210-x. [DOI] [PubMed] [Google Scholar]
  18. Shapiro B. M., Siccardi A. G., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. Membrane prtein alterations associated with mutations affecting the initiation of DNA synthesis. J Mol Biol. 1970 Aug 28;52(1):75–89. doi: 10.1016/0022-2836(70)90178-6. [DOI] [PubMed] [Google Scholar]
  19. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  20. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]
  21. Walker J. R. Thymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli. J Bacteriol. 1970 Dec;104(3):1391–1392. doi: 10.1128/jb.104.3.1391-1392.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES