Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Feb;113(2):907–913. doi: 10.1128/jb.113.2.907-913.1973

Metabolism of d-Fructose by Arthrobacter pyridinolis

Mark E Sobel 1, Terry A Krulwich 1
PMCID: PMC285308  PMID: 4347929

Abstract

Previous studies showed that Arthrobacter pyridinolis can transport and utilize d-glucose only after prior growth on certain Krebs cycle intermediates. In contrast, we found that d-fructose was taken up and metabolized by A. pyridinolis without special prior conditions of growth. d-Fructose was first converted to d-fructose-1-phosphate by a phosphoenolpyruvate (PEP):D-fructose phosphotransferase. This activity required both supernatant and pellet fractions from d-fructose-grown cells centrifuged at 150,000 × g. The d-fructose-1-phosphate formed was converted to d-fructose-1, 6-diphosphate. Mutants deficient in PEP:d-fructose phosphotransferase and d-fructose-1-phosphate kinase, or d-fructose-1, 6-diphosphatase (FDPase) were unable to grow on d-fructose but retained the normal ability to use d-glucose. Mutants forming reduced amounts of FDPase were completely unable to grow on d-fructose but were still capable of limited growth on Krebs cycle intermediates. A requirement for higher levels of FDPase for growth on d-fructose than for growth on Krebs cycle intermediates was also indicated by the higher specific activities of FDPase in d-fructose-grown cells than in cells grown on l-malate or amino acids.

Full text

PDF
907

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. L., Wood W. A. Carbohydrate metabolism in microorganisms. Annu Rev Microbiol. 1969;23:539–578. doi: 10.1146/annurev.mi.23.100169.002543. [DOI] [PubMed] [Google Scholar]
  2. EGAN J. B., MORSE M. L. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT. Biochim Biophys Acta. 1965 Feb 15;97:310–319. doi: 10.1016/0304-4165(65)90096-6. [DOI] [PubMed] [Google Scholar]
  3. Fraenkel D. G., Horecker B. L. Fructose-1, 6-diphosphatase and acid hexose phosphatase of Escherichia coli. J Bacteriol. 1965 Oct;90(4):837–842. doi: 10.1128/jb.90.4.837-842.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanson T. E., Anderson R. L. Phosphoenolpyruvate-dependent formation of D-fructose 1-phosphate by a four-component phosphotransferase system. Proc Natl Acad Sci U S A. 1968 Sep;61(1):269–276. doi: 10.1073/pnas.61.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaback H. R., Barnes E. M., Jr Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem. 1971 Sep 10;246(17):5523–5531. [PubMed] [Google Scholar]
  6. Kashket E. R., Wilson T. H. Role of metabolic energy in the transport of -galactosides by Streptococcus lactis. J Bacteriol. 1972 Feb;109(2):784–789. doi: 10.1128/jb.109.2.784-789.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kerwar G. K., Gordon A. S., Kaback H. R. Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. J Biol Chem. 1972 Jan 10;247(1):291–297. [PubMed] [Google Scholar]
  8. Kornberg H. L., Smith J. Role of phosphofructokinase in the utilization of glucose by Escherichia coli. Nature. 1970 Jul 4;227(5253):44–46. doi: 10.1038/227044a0. [DOI] [PubMed] [Google Scholar]
  9. Krulwich T. A., Ensign J. C. Alteration of glucose metabolism of Arthrobacter crystallopoietes by compounds which induce sphere to rod morphogenesis. J Bacteriol. 1969 Feb;97(2):526–534. doi: 10.1128/jb.97.2.526-534.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Nakashima K., Horecker B. L., Traniello S., Pontremoli S. Rabbit liver and rabbit kidney fructose diphosphatases: catalytic properties of enzymes activated by coenzyme A and acyl carrier protein. Arch Biochem Biophys. 1970 Jul;139(1):190–199. doi: 10.1016/0003-9861(70)90060-3. [DOI] [PubMed] [Google Scholar]
  12. Newsholme E. A., Robinson J., Taylor K. A radiochemical enzymatic activity assay for glycerol kinase and hexokinase. Biochim Biophys Acta. 1967 Mar 15;132(2):338–346. doi: 10.1016/0005-2744(67)90153-2. [DOI] [PubMed] [Google Scholar]
  13. Patni N. J., Alexander J. K. Catabolism of fructose and mannitol in Clostridium thermocellum: presence of phosphoenolpyruvate: fructose phosphotransferase, fructose 1-phosphate kinase, phosphoenolpyruvate: mannitol phosphotransferase, and mannitol 1-phosphate dehydrogenase in cell extracts. J Bacteriol. 1971 Jan;105(1):226–231. doi: 10.1128/jb.105.1.226-231.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patni N. J., Alexander J. K. Utilization of glucose by Clostridium thermocellum: presence of glucokinase and other glycolytic enzymes in cell extracts. J Bacteriol. 1971 Jan;105(1):220–225. doi: 10.1128/jb.105.1.220-225.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Romano A. H., Eberhard S. J., Dingle S. L., McDowell T. D. Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in bacteria. J Bacteriol. 1970 Nov;104(2):808–813. doi: 10.1128/jb.104.2.808-813.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sapico V., Hanson T. E., Walter R. W., Anderson R. L. Metabolism of D-fructose in Aerobacter aerogenes: analysis of mutants lacking D-fructose 6-phosphate kinase and D-fructose 1,6-diphosphatase. J Bacteriol. 1968 Jul;96(1):51–54. doi: 10.1128/jb.96.1.51-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simoni R. D., Smith M. F., Roseman S. Resolution of a staphylococcal phosphotransferase system into four protein components and its relation to sugar transport. Biochem Biophys Res Commun. 1968 Jun 10;31(5):804–811. doi: 10.1016/0006-291x(68)90634-7. [DOI] [PubMed] [Google Scholar]
  19. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  20. Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tanaka S., Lin E. C. Two classes of pleiotropic mutants of Aerobacter aerogenes lacking components of a phosphoenolpyruvate-dependent phosphotransferase system. Proc Natl Acad Sci U S A. 1967 Apr;57(4):913–919. doi: 10.1073/pnas.57.4.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wolfson P. J., Krulwich T. A. Inhibition of isocitrate lyase: the basis for inhibition of growth of two Arthrobacter species by pyruvate. J Bacteriol. 1972 Oct;112(1):356–364. doi: 10.1128/jb.112.1.356-364.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES