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. 1973 Feb;113(2):1006–1014. doi: 10.1128/jb.113.2.1006-1014.1973

Mechanism for the Regulation of Cell Division in Agmenellum

L O Ingram a,1, W D Fisher a
PMCID: PMC285319  PMID: 4632309

Abstract

We describe a nonlethal temperature-conditional mutant of Agmenellum quadruplicatum which allows dissociation of the processes of growth and cell division. With this system, evidence has been obtained for the regulation of one step in the process of cell division by a small effector molecule. The effector molecule is apparently released into the surrounding medium and can be obtained from lyophilized spent medium by extraction with 80% ethanol. The addition of this extract to serpentine filaments of the SN29 mutant strain stimulates cell division in these filaments and leads to the production of cells approximating normal dimensions within one generation time. The degree of stimulation of cell division is directly related to the amount of extract added. A general hypothesis is presented for the positive regulation of the initiation of cell wall and cell membrane invagination in this organism.

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Selected References

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  1. ADLER H. I., HARDIGREE A. A. POSTIRRADIATION GROWTH, DIVISION, AND RECOVERY IN BACTERIA. Radiat Res. 1965 May;25:92–102. [PubMed] [Google Scholar]
  2. Adler H. I., Fisher W. D., Hardigree A. A., Stapleton G. E. Repair of radiation-induced damage to the cell division mechanism of Escherichia coli. J Bacteriol. 1966 Feb;91(2):737–742. doi: 10.1128/jb.91.2.737-742.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Byers B. R., Powell M. V., Lankford C. E. Iron-chelating hydroxamic acid (schizokinen) active in initiation of cell division in Bacillus megaterium. J Bacteriol. 1967 Jan;93(1):286–294. doi: 10.1128/jb.93.1.286-294.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. J. Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r. J Bacteriol. 1968 Oct;96(4):1214–1224. doi: 10.1128/jb.96.4.1214-1224.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GRULA E. A., GRULA M. M. Cell division in a species of Erwinia III. Reversal of inhibition of cell division caused by D-amino acids, penicillin, and ultraviolet light. J Bacteriol. 1962 May;83:981–988. doi: 10.1128/jb.83.5.981-988.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grula E. A., Hopfer R. L. Cell division in a species of Erwinia. 13. Leakage of proteins from the peripheral area of filamentous cells. Biochim Biophys Acta. 1972 Mar 17;255(3):822–832. doi: 10.1016/0005-2736(72)90394-x. [DOI] [PubMed] [Google Scholar]
  7. Grula E. A., King R. D. Changes in the cell membranes of dividing and non-dividing cells of Micrococcus lysodeikticus disIIp + . Biochem Biophys Res Commun. 1971 Sep 17;44(6):1356–1363. doi: 10.1016/s0006-291x(71)80235-8. [DOI] [PubMed] [Google Scholar]
  8. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  10. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  11. Huzyk L., Clark D. J. Nucleoside triphosphate pools in synchronous cultures of Escherichia coli. J Bacteriol. 1971 Oct;108(1):74–81. doi: 10.1128/jb.108.1.74-81.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ingram L. O., Fisher W. D. Novel mutant impaired in cell division: evidence for a positive regulating factor. J Bacteriol. 1973 Feb;113(2):999–1005. doi: 10.1128/jb.113.2.999-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ingram L. O., Thurston E. L., Van Baalen C. Effects of selected inhibitors on growth and cell division in Agmenellum. Arch Mikrobiol. 1972;81(1):1–12. doi: 10.1007/BF00715019. [DOI] [PubMed] [Google Scholar]
  14. Ingram L. O., Van Baalen C. Characteristics of a stable, filamentous mutant of a coccoid blue-green alga. J Bacteriol. 1970 Jun;102(3):784–789. doi: 10.1128/jb.102.3.784-789.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inouye M., Pardee A. B. Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli. J Biol Chem. 1970 Nov 10;245(21):5813–5819. [PubMed] [Google Scholar]
  16. Inouye M., Pardee A. B. Requirement of polyamines for bacterial division. J Bacteriol. 1970 Mar;101(3):770–776. doi: 10.1128/jb.101.3.770-776.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Inouye M. Reversal by sodium chloride of envelope protein changes related to DNA replication and cell division of Escherichia coli. J Mol Biol. 1972 Feb 14;63(3):597–600. doi: 10.1016/0022-2836(72)90451-2. [DOI] [PubMed] [Google Scholar]
  18. KOCH A. L., SCHAECHTER M. A model for statistics of the cell division process. J Gen Microbiol. 1962 Nov;29:435–454. doi: 10.1099/00221287-29-3-435. [DOI] [PubMed] [Google Scholar]
  19. Kennell D., Kotoulas A. Magnesium starvation of Aerobacter aerogenes. IV. Cytochemical changes. J Bacteriol. 1967 Jan;93(1):367–378. doi: 10.1128/jb.93.1.367-378.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kojima M., Suda S., Hotta S., Hamada K., Suganuma A. Necessity of calcium ion for cell division in Lactobacillus bifidus. J Bacteriol. 1970 Nov;104(2):1010–1013. doi: 10.1128/jb.104.2.1010-1013.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LOVELESS L. E., SPOERL E., WEISMAN T. H. A survey of effects of chemicals on division and growth of yeast and Escherichia coli. J Bacteriol. 1954 Dec;68(6):637–644. doi: 10.1128/jb.68.6.637-644.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Montgomerie J. Z., Potter C. S., Kalmanson G. M., Guze L. B. Penicillin: effect on sodium and potassium transport in bacterial and protoplast forms of Sterptococcus faecalis. Science. 1968 Mar 15;159(3820):1252–1254. doi: 10.1126/science.159.3820.1252. [DOI] [PubMed] [Google Scholar]
  23. Pierucci O., Helmstetter C. E. Chromosome replication, protein synthesis and cell division in Escherichia coli. Fed Proc. 1969 Nov-Dec;28(6):1755–1760. [PubMed] [Google Scholar]
  24. Ricard M., Hirota Y. Effet des sels sur le processus de division cellulaire d'E. coli. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1335–1338. [PubMed] [Google Scholar]
  25. Ruff W., Kirby E. P., Goldthwait D. A. Cell division and prophage induction in Escherichia coli: studies of nucleotide levels. J Bacteriol. 1971 Jun;106(3):994–1004. doi: 10.1128/jb.106.3.994-1004.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sivak A., Mossman B. T., Van Duuren B. L. Activation of cell membrane enzymes in the stimulation of cell division. Biochem Biophys Res Commun. 1972 Jan 31;46(2):605–609. doi: 10.1016/s0006-291x(72)80182-7. [DOI] [PubMed] [Google Scholar]
  27. Smith H. S., Pardee A. B. Accumulation of a protein required for division during the cell cycle of Escherichia coli. J Bacteriol. 1970 Mar;101(3):901–909. doi: 10.1128/jb.101.3.901-909.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stárka J. Cell envelope proteins of dividing and non-dividing cells of Escherichia coli. FEBS Lett. 1971 Aug 15;16(3):223–225. doi: 10.1016/0014-5793(71)80139-4. [DOI] [PubMed] [Google Scholar]
  29. Stárka J., Moravová J. Phospholipids and cellular division of Escherichia coli. J Gen Microbiol. 1970 Feb;60(2):251–257. doi: 10.1099/00221287-60-2-251. [DOI] [PubMed] [Google Scholar]
  30. Tamás G., Tarján I., Szógyi M. Effect of antibiotics on the ion-exchange of bacteria. Acta Biochim Biophys Acad Sci Hung. 1971;6(2):173–178. [PubMed] [Google Scholar]
  31. Van Alstyne D., Simon M. I. Division mutants of Bacillus subtilis: isolation and PBS1 transduction of division-specific markers. J Bacteriol. 1971 Dec;108(3):1366–1379. doi: 10.1128/jb.108.3.1366-1379.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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