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. 1973 Feb;113(2):1081–1083. doi: 10.1128/jb.113.2.1081-1083.1973

Screening Procedure for Escherichia coli Mutants Deficient in Ribonuclease I

S Kaplan 1
PMCID: PMC285334  PMID: 4570595

Abstract

Strains of Escherichia coli that possess ribonucleic acid accumulated under relaxed growth conditions show a considerable increase in time before the onset of β-galactosidase inducibility. This time dependency can be related to the presence or absence of ribonuclease I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrell J. W. Ribonuclease I released from Escherichia coli by osmotic shock. Arch Biochem Biophys. 1971 Feb;142(2):693–700. doi: 10.1016/0003-9861(71)90535-2. [DOI] [PubMed] [Google Scholar]
  2. BOREK E., ROCKENBACH J., RYAN A. Studies on a mutant of Escherichia coli with unbalanced ribonucleic acid synthesis. J Bacteriol. 1956 Mar;71(3):318–323. doi: 10.1128/jb.71.3.318-323.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ben-Hamida F., Schlessinger D. Synthesis and breakdown of ribonucleic acid in Escherichia coli starving for nitrogen. Biochim Biophys Acta. 1966 Apr 18;119(1):183–191. doi: 10.1016/0005-2787(66)90049-9. [DOI] [PubMed] [Google Scholar]
  4. Dalgarno L., Gros F. RNA synthesis in "relaxed" and "stringent" Escherichia coli. Breakdown of performed ribonucleoprotein particles and subsequent RNA synthesis. Biochim Biophys Acta. 1968 Mar 18;157(1):64–75. [PubMed] [Google Scholar]
  5. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  6. Fiil N., Friesen J. D. Isolation of "relaxed" mutants of Escherichia coli. J Bacteriol. 1968 Feb;95(2):729–731. doi: 10.1128/jb.95.2.729-731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  8. KURLAND C. G., NOMURA M., WATSON J. D. The physical properties of the chloromycetin particles. J Mol Biol. 1962 May;4:388–394. doi: 10.1016/s0022-2836(62)80019-9. [DOI] [PubMed] [Google Scholar]
  9. Kaplan S., Anderson D. Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J Bacteriol. 1968 Mar;95(3):991–997. doi: 10.1128/jb.95.3.991-997.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NEIDHARDT F. C., EIDLIC L. Characterization of the RNA formed under conditions of relaxed amino acid control in Escherichia coli. Biochim Biophys Acta. 1963 Mar 26;68:380–388. doi: 10.1016/0006-3002(63)90159-8. [DOI] [PubMed] [Google Scholar]
  11. NEU H. C., HEPPEL L. A. THE RELEASE OF RIBONUCLEASE INTO THE MEDIUM WHEN ESCHERICHIA COLI CELLS ARE CONVERTED TO SPEROPLASTS. J Biol Chem. 1964 Nov;239:3893–3900. [PubMed] [Google Scholar]
  12. Natori S., Mizuno D. Turnover of ribosomal RNA in a ribonuclease I-less mutant of Escherichia coli, Q-13, which was found to possess polynucleotide phosphorylase. Biochim Biophys Acta. 1967 Sep 26;145(2):328–336. doi: 10.1016/0005-2787(67)90051-2. [DOI] [PubMed] [Google Scholar]
  13. Reiner A. M. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. J Bacteriol. 1969 Mar;97(3):1431–1436. doi: 10.1128/jb.97.3.1431-1436.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SYPHERD P. S., STRAUSS N. THE ROLE OF RNA IN REPRESSION OF ENZYME SYNTHESIS. Proc Natl Acad Sci U S A. 1963 Dec;50:1059–1066. doi: 10.1073/pnas.50.6.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tam Lennette E., Meyhack B., Apirion D. A mutation affecting degradation of stable RNA in Escherichia coli. FEBS Lett. 1972 Apr 1;21(3):286–288. doi: 10.1016/0014-5793(72)80184-4. [DOI] [PubMed] [Google Scholar]
  16. YANAGISAWA K. Preferential synthesis of beta-galactosidase after amino acid starvation. Biochem Biophys Res Commun. 1962 Sep 25;9:84–87. doi: 10.1016/0006-291x(62)90092-x. [DOI] [PubMed] [Google Scholar]
  17. YANAGISAWA K. The simultaneous accumulation of RNA and of a repressor of beta-galactosidase synthesis. Biochem Biophys Res Commun. 1962 Sep 25;9:88–93. doi: 10.1016/0006-291x(62)90093-1. [DOI] [PubMed] [Google Scholar]

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