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. 1973 Jun;114(3):916–927. doi: 10.1128/jb.114.3.916-927.1973

Polynucleotide Sequence Relationships Among Japanese and American Strains of Vibrio parahaemolyticus

T E Staley 1, R R Colwell 1
PMCID: PMC285345  PMID: 4712574

Abstract

Polynucleotide sequence relationships between two reference Vibrio parahaemolyticus strains isolated from Japanese and American gastroenteritis patients were investigated by use of 32P-DNA/DNA reassociation in free solution. In addition, these strains were similarly compared with 22 other strains of estuarine and marine vibrios, including 11 strains previously identified as V. parahaemolyticus (2 Japanese, 1 of unknown location, and 8 American strains obtained from diverse geographical locations and sources in North America), 3 strains of V. alginolyticus, and 8 of Vibrio spp. Deoxyribonucleic acid (DNA) from the Japanese and American gastroenteritis isolates showed high relative levels of intraspecific duplex formation (92 to 93%) when reassociated, reciprocally, at 60 C. Heterologous DNA duplexes exhibited thermal elution midpoint [Tm(e)] values comparable to those obtained from homologous duplexes (88.0) when thermally eluted from hydroxyapatite, thus indicating high base-pair complementarity. Other V. parahaemolyticus strains showed DNA homologies of 85% or greater, with correspondingly high Tm(e) values (86.0 to 88.0) for the heteroduplexes formed. DNA of two of three V. alginolyticus strains (ATCC 17749 and 166-70) was 55 to 60% homologous to reference V. parahaemolyticus DNA preparations; Vibrio sp. strain 5144 (originally classified as V. parahaemolyticus biotype 2 and subsequently as V. alginolyticus strain 5144) showed only 24 to 26% DNA homology to the same reference DNA. These data provide evidence that Vibrio sp. strain 5144 is genetically distinct from the other V. alginolyticus strains used in this study. Three bioluminescent strains thought to be closely related to V. parahaemolyticus demonstrated only 24 to 31% DNA homology to the reference V. parahaemolyticus DNA. These data firmly establish the existence in some Atlantic and Gulf Coast estuaries of organisms genetically very similar to V. parahaemolyticus, the causative agent of “shirasu” food poisoning in Japan.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. S., Ordal E. J. Deoxyribonucleic acid relationships among marine vibrios. J Bacteriol. 1972 Feb;109(2):696–706. doi: 10.1128/jb.109.2.696-706.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker W. H., Jr, Hooper D., Baross J. A. Shellfish-related gastroenteritis. N Engl J Med. 1970 Aug 6;283(6):319–319. [PubMed] [Google Scholar]
  3. Baross J., Liston J. Isolation of vibrio parahaemolyticus from the Northwest Pacific. Nature. 1968 Mar 30;217(5135):1263–1264. doi: 10.1038/2171263a0. [DOI] [PubMed] [Google Scholar]
  4. Baross J., Liston J. Occurrence of Vibrio parahaemolyticus and related hemolytic vibrios in marine environments of Washington State. Appl Microbiol. 1970 Aug;20(2):179–186. doi: 10.1128/am.20.2.179-186.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barrow G. I., Miller D. C. Vibrio parahaemolyticus: a potential pathogen from marine sources in Britain. Lancet. 1972 Feb 26;1(7748):485–486. doi: 10.1016/s0140-6736(72)90134-1. [DOI] [PubMed] [Google Scholar]
  6. Bartley C. H., Slanetz L. W. Occurrence of Vibrio parahaemolyticus in estuarine waters and oysters of New Hampshire. Appl Microbiol. 1971 May;21(5):965–966. doi: 10.1128/am.21.5.965-966.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Battey Y. M., Wallace R. B., Allan B. C., Keeffe B. M. Gastro-enteritis in Australia caused by a marine vibrio. Med J Aust. 1970 Feb 28;1(9):430–433. doi: 10.5694/j.1326-5377.1970.tb77967.x. [DOI] [PubMed] [Google Scholar]
  8. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. Polynucleotide sequence relationships among members of Enterobacteriaceae. J Bacteriol. 1969 May;98(2):637–650. doi: 10.1128/jb.98.2.637-650.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. Batch procedure for thermal elution of DNA from hydroxyapatite. Anal Biochem. 1969 Apr 4;28(1):447–459. doi: 10.1016/0003-2697(69)90199-7. [DOI] [PubMed] [Google Scholar]
  10. Brenner D. J., Fanning G. R., Skerman F. J., Falkow S. Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms. J Bacteriol. 1972 Mar;109(3):953–965. doi: 10.1128/jb.109.3.953-965.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Citarella R. V., Colwell R. R. Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J Bacteriol. 1970 Oct;104(1):434–442. doi: 10.1128/jb.104.1.434-442.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Colwell R. R. Polyphasic taxonomy of the genus vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J Bacteriol. 1970 Oct;104(1):410–433. doi: 10.1128/jb.104.1.410-433.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fishbein M., Mehlman I. J., Pitcher J. Isolation of Vibrio parahaemolyticus from the processed meat of Chesapeake Bay blue crabs. Appl Microbiol. 1970 Aug;20(2):176–178. doi: 10.1128/am.20.2.176-178.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hanaoka M., Kato Y., Amano T. Complementary examination of DNA'S among Vibrio species. Biken J. 1969 Sep;12(3):181–185. [PubMed] [Google Scholar]
  15. Johnson J. L., Ordal E. J. Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. J Bacteriol. 1968 Mar;95(3):893–900. doi: 10.1128/jb.95.3.893-900.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krantz G. E., Colwell R. R., Lovelace E. Vibrio parahaemolyticus from the blue crab Callinectes sapidus in Chesapeake Bay. Science. 1969 Jun 13;164(3885):1286–1287. doi: 10.1126/science.164.3885.1286. [DOI] [PubMed] [Google Scholar]
  17. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  18. Molenda J. R., Johnson W. G., Fishbein M., Wentz B., Mehlman I. J., Dadisman T. A., Jr Vibrio parahaemolyticus gastroenteritis in Maryland: laboratory aspects. Appl Microbiol. 1972 Sep;24(3):444–448. doi: 10.1128/am.24.3.444-448.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roland F. P. Leg gangrene and endotoxin shock due to vibrio parahaemolyticus--an infection acquired in New England coastal waters. N Engl J Med. 1970 Jun 4;282(23):1306–1306. doi: 10.1056/NEJM197006042822306. [DOI] [PubMed] [Google Scholar]
  20. SAKAZAKI R., IWANAMI S., FUKUMI H. STUDIES ON THE ENTEROPATHOGENIC, FACULTATIVELY HALOPHILIC BACTERIA, VIBRIO PARAHAEMOLYTICUS. I. MORPHOLOGICAL, CULTURAL AND BIOCHEMICAL PROPERTIES AND ITS TAXONOMICAL POSITION. Jpn J Med Sci Biol. 1963 Aug;16:161–188. doi: 10.7883/yoken1952.16.161. [DOI] [PubMed] [Google Scholar]
  21. Sakazaki R. Proposal of Vibrio alginolyticus for the biotype 2 of Vibrio parahaemolyticus. Jpn J Med Sci Biol. 1968 Oct;21(5):359–362. doi: 10.7883/yoken1952.21.359. [DOI] [PubMed] [Google Scholar]
  22. Skerman F. J., Formal S. B., Falkow S. Plasmid-associated enterotoxin production in a strain of Escherichia coli isolated from humans. Infect Immun. 1972 Apr;5(4):622–624. doi: 10.1128/iai.5.4.622-624.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thomson W. K., Trenholm D. A. The isolation of Vibrio parahaemolyticus and related halophilic bacteria from Canadian Atlantic shellfish. Can J Microbiol. 1971 Apr;17(4):545–549. doi: 10.1139/m71-089. [DOI] [PubMed] [Google Scholar]
  24. Twedt R. M., Spaulding P. L., Hall H. E. Morphological, cultural, biochemical, and serological comparison of Japanese strains of Vibrio parahemolyticus with related cultures isolated in the United States. J Bacteriol. 1969 May;98(2):511–518. doi: 10.1128/jb.98.2.511-518.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vanderzant C., Nickelson R. Procedure for isolation and enumeration of Vibrio parahaemolyticus. Appl Microbiol. 1972 Jan;23(1):26–33. doi: 10.1128/am.23.1.26-33.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ward B. Q. Isolations of organisms related to Vibrio parahemolyticus from American estuarine sediments. Appl Microbiol. 1968 Mar;16(3):543–546. doi: 10.1128/am.16.3.543-546.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zen-Yoji H., Sakai S., Terayama T., Kudo Y., Ito T., Benoki M., Nagasaki M. Epidemiology, enteropathogenicity, and classification of Vi.rio parahaemolyticus. J Infect Dis. 1965 Dec;115(5):436–444. doi: 10.1093/infdis/115.5.436. [DOI] [PubMed] [Google Scholar]

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