Abstract
A stabilized L-form of Streptococcus pyogenes continues to synthesize glycerol teichoic acid. This polymer was obtained from S. pyogenes and its L-form, treated in identical fashion, and compared. Highly purified glycerol teichoic acid from only the L-form was found to be devoid of d-alanine and to have a shorter chain length. Otherwise, the glycerol teichoic acid from these two organisms was found to be a 1,3-phosphodiester-linked glycerophosphate polymer substituted with d-glucose. Evidence is presented that most, if not all, of the glycerol teichoic acid in this streptococcus lies between the wall and membrane. A possible need for the continued synthesis of a minute amount of glycerol teichoic acid by this L-form for survival is discussed in terms of the known function of teichoic acids in bacteria.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Archibald A. R., Baddiley J., Blumsom N. L. The teichoic acids. Adv Enzymol Relat Areas Mol Biol. 1968;30:223–253. doi: 10.1002/9780470122754.ch5. [DOI] [PubMed] [Google Scholar]
- Archibald A. R., Baddiley J., Button D. The membrane teichoic acid of Staphylococcus lactis I3. Biochem J. 1968 Dec;110(3):559–563. doi: 10.1042/bj1100559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Archibald A. R., Baddiley J. The teichoic acids. Adv Carbohydr Chem Biochem. 1966;21:323–375. doi: 10.1016/s0096-5332(08)60320-3. [DOI] [PubMed] [Google Scholar]
- BADDILEY J., BUCHANAN J. G., RAJBHANDARY U. L., SANDERSON A. R. Teichoic acid from the walls of Staphylococcus aureus H. Structure of the N-acetylglucosaminyl-ribitol residues. Biochem J. 1962 Mar;82:439–448. doi: 10.1042/bj0820439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRITCHLEY P., ARCHIBALD A. R., BADDILEY J. The intracellular teichoic acid from Lactobacillus arabinosus 17-5. Biochem J. 1962 Dec;85:420–431. doi: 10.1042/bj0850420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen M., Panos C. Cell wall polysaccharide biosynthesis by membrane fragments from Streptococcus pyogenes and stabilized L-form. J Bacteriol. 1971 May;106(2):347–355. doi: 10.1128/jb.106.2.347-355.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
- Edwards J., Panos C. STREPTOCOCCAL L FORMS V. : Acid-Soluble Nucleotides of a Group A Streptococcus and Derived L Form. J Bacteriol. 1962 Dec;84(6):1202–1208. doi: 10.1128/jb.84.6.1202-1208.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellwood D. C., Kelemen M. V., Baddiley J. The glycerol teichoic acid from the walls of Staphylococcus albus N.T.C.C. 7944. Biochem J. 1963 Feb;86(2):213–225. doi: 10.1042/bj0860213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANAHAN D. J., OLLEY J. N. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- HIJMANS W. Absence of the group-specific and the cell-wall polysaccharide antigen in L-phase variants of group D streptococci. J Gen Microbiol. 1962 Apr;28:177–179. doi: 10.1099/00221287-28-1-177. [DOI] [PubMed] [Google Scholar]
- Hughes A. H., Stow M., Hancock I. C., Baddiley J. Function of teichoic acids and effect of novobiocin on control of Mg2+ at the bacterial membrane. Nat New Biol. 1971 Jan 13;229(2):53–55. doi: 10.1038/newbio229053a0. [DOI] [PubMed] [Google Scholar]
- KELEMEN M. V., BADDILEY J. Structure of the intracellular glycerol teichoic acid from Lactobacillus casei A.T.C.C. 7469. Biochem J. 1961 Aug;80:246–254. doi: 10.1042/bj0800246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCCARTY M. THE ROLE OF D-ALANINE IN THE SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL GLYCEROL TEICHOIC ACID. Proc Natl Acad Sci U S A. 1964 Aug;52:259–265. doi: 10.1073/pnas.52.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsuno T., Slade H. D. Composition and properties of a group A streptococcal teichoic acid. J Bacteriol. 1970 Jun;102(3):747–752. doi: 10.1128/jb.102.3.747-752.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mauck J., Chan L., Glaser L. Turnover of the cell wall of Gram-positive bacteria. J Biol Chem. 1971 Mar 25;246(6):1820–1827. [PubMed] [Google Scholar]
- McCARTY M. The occurrence of polyglycerophosphate as an antigenic component of various gram-positive bacterial species. J Exp Med. 1959 Apr 1;109(4):361–378. doi: 10.1084/jem.109.4.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
- Pandhi P. N., Panos C. Biosynthesis of D-alanyl-D-alanine from L-alanine by extracts of a stabilized L-form from Streptococcus pyogenes. J Gen Microbiol. 1972 Aug;71(3):487–494. doi: 10.1099/00221287-71-3-487. [DOI] [PubMed] [Google Scholar]
- Panos C., Cohen M., Fagan G. Antibiotic inhibition and binding studies with a group A streptococcal L-form. J Gen Microbiol. 1967 Feb;46(2):299–304. doi: 10.1099/00221287-46-2-299. [DOI] [PubMed] [Google Scholar]
- Panos C., Fagan G., Zarkadas C. G. Comparative electrophoretic and amino acid analyses of isolated membranes from Streptococcus pyogenes and stabilized L-form. J Bacteriol. 1972 Oct;112(1):285–290. doi: 10.1128/jb.112.1.285-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pratt B. C. Cell-wall deficiencies in L-forms of Staphylococcus aureus. J Gen Microbiol. 1966 Jan;42(1):115–122. doi: 10.1099/00221287-42-1-115. [DOI] [PubMed] [Google Scholar]
- ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]
- Reusch V. M., Jr, Neuhaus F. C. D-Alanine: membrane acceptor ligase from Lactobacillus casei. J Biol Chem. 1971 Oct 25;246(20):6136–6143. [PubMed] [Google Scholar]
- SMITH D. G., SHATTOCK P. M. THE CELLULAR LOCATION OF ANTIGENS IN STREPTOCOCCI OF GROUPS D, N AND Q. J Gen Microbiol. 1964 Jan;34:165–175. doi: 10.1099/00221287-34-1-165. [DOI] [PubMed] [Google Scholar]
- Shaw N., Baddiley J. The teichoic acid from the walls of Lactobacillus buchneri N.C.I.B. 8007. Biochem J. 1964 Nov;93(2):317–321. doi: 10.1042/bj0930317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
- WICKEN A. J., ELLIOTT S. D., BADDILEY J. The identity of streptococcal group D antigen with teichoic acid. J Gen Microbiol. 1963 May;31:231–239. doi: 10.1099/00221287-31-2-231. [DOI] [PubMed] [Google Scholar]
- Watkinson R. J., Hussey H., Baddiley J. Shared lipid phosphate carrier in the biosynthesis of teichoic acid and peptidoglycan. Nat New Biol. 1971 Jan 13;229(2):57–59. doi: 10.1038/newbio229057a0. [DOI] [PubMed] [Google Scholar]