Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Jun;114(3):974–979. doi: 10.1128/jb.114.3.974-979.1973

Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida

N W Dunn 1, I C Gunsalus 1
PMCID: PMC285353  PMID: 4712575

Abstract

The capacity of Pseudomonas putida PpG7 (ATCC 17,485) to grow on naphthalene, phenotype Nah+, is lost spontaneously, and the frequency is increased by treatment with mitomycin C. The Nah+ growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. After mutagenesis, strains can be selected with increased donor capacity in conjugation. Clones which use naphthalene grow on salicylate and carry catechol 2,3-oxygenase, the initial enzyme of the aromatic α-keto acid pathway, whereas cured strains grow neither on salicylate nor naphthalene and lack catechol 2,3-oxygenase, but retain catechol 1,2-oxygenase and the aromatic β-keto adipate pathway enzymes.

Full text

PDF
974

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chakrabarty A. M. Genetic basis of the biodegradation of salicylate in Pseudomonas. J Bacteriol. 1972 Nov;112(2):815–823. doi: 10.1128/jb.112.2.815-823.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chakrabarty A. M., Gunsalus C. F., Gunsalus I. C. Transduction and the clustering of genes in fluorescent Pseudomonads. Proc Natl Acad Sci U S A. 1968 May;60(1):168–175. doi: 10.1073/pnas.60.1.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chakrabarty A. M., Gunsalus I. C. Defective phage and chromosome mobilization in Pseudomonas putida. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1217–1223. doi: 10.1073/pnas.64.4.1217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chakrabarty A. M., Gunsalus I. C. Transduction and genetic homology between Pseudomonas species putida and aeruginosa. J Bacteriol. 1970 Sep;103(3):830–832. doi: 10.1128/jb.103.3.830-832.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAGLEY S., EVANS W. C., RIBBONS D. W. New pathways in the oxidative metabolism of aromatic compounds by microorganisms. Nature. 1960 Nov 12;188:560–566. doi: 10.1038/188560a0. [DOI] [PubMed] [Google Scholar]
  6. FARGIE B., HOLLOWAY B. W. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA. Genet Res. 1965 Jul;6:284–299. doi: 10.1017/s0016672300004158. [DOI] [PubMed] [Google Scholar]
  7. Feist C. F., Hegeman G. D. Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. J Bacteriol. 1969 Nov;100(2):869–877. doi: 10.1128/jb.100.2.869-877.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gunsalus C., Gunsalus C. F., Chakrabarty A. M., Sikes S., Crawford I. P. Fine structure mapping of the tryptophan genes in Pseudomonas putida. Genetics. 1968 Nov;60(3):419–435. doi: 10.1093/genetics/60.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hegeman G. D. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol. 1966 Mar;91(3):1140–1154. doi: 10.1128/jb.91.3.1140-1154.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jacobson L. A., Bartholomaus R. C., Gunsalus I. C. Repression of malic enzyme by acetate in Pseudomonas. Biochem Biophys Res Commun. 1966 Sep 22;24(6):955–960. doi: 10.1016/0006-291x(66)90343-3. [DOI] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. Ornston L. N. Regulation of catabolic pathways in Pseudomonas. Bacteriol Rev. 1971 Jun;35(2):87–116. doi: 10.1128/br.35.2.87-116.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rheinwald J. G., Chakrabarty A. M., Gunsalus I. C. A transmissible plasmid controlling camphor oxidation in Pseudomonas putida. Proc Natl Acad Sci U S A. 1973 Mar;70(3):885–889. doi: 10.1073/pnas.70.3.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  15. Wheelis M. L., Stanier R. Y. The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics. 1970 Oct;66(2):245–266. doi: 10.1093/genetics/66.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wu C. H., Ornston M. K., Ornston L. N. Genetic control of enzyme induction in the -ketoadipate pathway of Pseudomonas putida: two-point crosses with a regulatory mutant strain. J Bacteriol. 1972 Feb;109(2):796–802. doi: 10.1128/jb.109.2.796-802.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES