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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Jun;114(3):1007–1013. doi: 10.1128/jb.114.3.1007-1013.1973

Regulation of Methionyl-Transfer Ribonucleic Acid Synthetase Formation in Escherichia coli and Salmonella typhimurium

Errol R Archibold 1, L S Williams 1
PMCID: PMC285357  PMID: 4576394

Abstract

The control of methionyl-transfer ribonucleic acid (tRNA) synthetase (l-methionine: soluble RNA ligase [adenosine monophosphate]) was studied in methionyl-tRNA synthetase mutants of Escherichia coli and Salmonella typhimurium. The results of activity determinations with crude extracts indicate that this enzyme of the E. coli mutant strain possessed a reduced affinity for methionine-tRNA, whereas this enzyme of the S. typhimurium mutant exhibited a decreased affinity for l-methionine. The differential rate of methionyl-tRNA synthetase formation in these two mutants was several-fold greater than that of the respective parental strains. On the other hand, the level of in vivo aminoacylation of methionine-tRNA was only about one-third that of the parent strains. These results suggest that aminoacylation of methionine-tRNA is a necessary step in repression control of methionyl-tRNA synthetase of both E. coli and S. typhimurium strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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