Abstract
Three branched-chain hydroxy acids not previously reported in other bacteria were found in extracts from saponified whole cells of Pseudomonas maltophilia. On the basis of evidence from mass spectrometry, infrared spectroscopy, and gas chromatographic procedures, they were identified as 2-hydroxy-9-methyldecanoic acid, 3-hydroxy-9-methyldecanoic acid, and 3-hydroxy-11-methyldodecanoic acid. These acids appeared to be tightly bound to other cellular components since they were not extracted from lyophilized cells with a chloroform-methanol (3:1) mixture.
Full text
PDF![1018](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/cd3a04acf8f8/jbacter00577-0132.png)
![1019](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/aa34676b1841/jbacter00577-0133.png)
![1020](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/b2943a20e320/jbacter00577-0134.png)
![1021](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/24568ce8faba/jbacter00577-0135.png)
![1022](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/fb3400704a79/jbacter00577-0136.png)
![1023](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/78eee07cfac5/jbacter00577-0137.png)
![1024](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34a/285359/fa0997f9f8ce/jbacter00577-0138.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bishop D. G., Hewett M. J., Knox K. W. Occurrence of 3-hydroxytridecanoic and 3-hydroxypentadecanoic acids in the lipopolysaccharides of Veillonella. Biochim Biophys Acta. 1971 Mar 16;231(2):274–276. doi: 10.1016/0005-2760(71)90138-x. [DOI] [PubMed] [Google Scholar]
- Brian B. L., Gardner E. W. A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Appl Microbiol. 1968 Apr;16(4):549–552. doi: 10.1128/am.16.4.549-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fensom A. H., Gray G. W. The chemical composition of the lipopolyacarideof Pseudomonas aeruginosa. Biochem J. 1969 Sep;114(2):185–196. doi: 10.1042/bj1140185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hancock I. C., Humphreys G. O., Meadow P. M. Characterisation of the hydroxy acids of Pseudomonas aeruginosa 8602. Biochim Biophys Acta. 1970 Mar 10;202(2):389–391. doi: 10.1016/0005-2760(70)90204-3. [DOI] [PubMed] [Google Scholar]
- Humphreys G. O., Hancock I. C., Meadow P. M. Synthesis of the hydroxyacids in lipid A of Pseudomonas aeruginosa. J Gen Microbiol. 1972 Jul;71(2):221–230. doi: 10.1099/00221287-71-2-221. [DOI] [PubMed] [Google Scholar]
- Key B. A., Gray G. W., Wilkinson S. G. The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes. Biochem J. 1970 Dec;120(3):559–566. doi: 10.1042/bj1200559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knoche H. W., Shively J. M. The identification of cis-11,12-methylene-2-hydroxyoctadecanoic acid from Thiobacillus thiooxidans. J Biol Chem. 1969 Sep 10;244(17):4773–4778. [PubMed] [Google Scholar]
- Moss C. W., Kellogg D. S., Jr, Farshy D. C., Lambert M. A., Thayer J. D. Cellular fatty acids of pathogenic Neisseria. J Bacteriol. 1970 Oct;104(1):63–68. doi: 10.1128/jb.104.1.63-68.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Lewis V. J. Characterization of clostridia by gas chromatography. I. Differentiation of species by cellular fatty acids. Appl Microbiol. 1967 Mar;15(2):390–397. doi: 10.1128/am.15.2.390-397.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Samuels S. B., Weaver R. E. Cellular fatty acid composition of selected Pseudomonas species. Appl Microbiol. 1972 Oct;24(4):596–598. doi: 10.1128/am.24.4.596-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RYHAGE R., STENHAGEN E. Mass spectrometry in lipid research. J Lipid Res. 1960 Oct;1:361–390. [PubMed] [Google Scholar]
- Tyrrell D. The fatty acid composition of some entomophthoraceae : II. The occurrence of branched-chain fatty acids inConidiobolus denaesporus Drechsl. Lipids. 1968 Jul;3(4):368–372. doi: 10.1007/BF02530941. [DOI] [PubMed] [Google Scholar]