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. 1973 Jun;114(3):1213–1216. doi: 10.1128/jb.114.3.1213-1216.1973

Measurement of the Cyanophycin Granule Polypeptide Contained in the Blue-Green Alga Anabaena cylindrica

Robert D Simon 1
PMCID: PMC285384  PMID: 4197270

Abstract

An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the cyanophycin granule is a cellular nitrogen reserve.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeliovich A., Shilo M. Photooxidative reactions of c-phycocyanin. Biochim Biophys Acta. 1972 Dec 14;283(3):483–491. doi: 10.1016/0005-2728(72)90264-2. [DOI] [PubMed] [Google Scholar]
  2. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen M. M., Smith A. J. Nitrogen chlorosis in blue-green algae. Arch Mikrobiol. 1969;69(2):114–120. doi: 10.1007/BF00409755. [DOI] [PubMed] [Google Scholar]
  4. Chao L., Bowen C. C. Purification and properties of glycogen isolated from a blue-green alga, Nostoc muscorum. J Bacteriol. 1971 Jan;105(1):331–338. doi: 10.1128/jb.105.1.331-338.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones D. D., Jost M. Isolation and chemical characterization of gas-vacuole membranes from Microcystis aeruginosa Kuetz. emend. Elenkin. Arch Mikrobiol. 1970;70(1):43–64. doi: 10.1007/BF00691059. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lang N. J. The fine structure of blue-green algae. Annu Rev Microbiol. 1968;22:15–46. doi: 10.1146/annurev.mi.22.100168.000311. [DOI] [PubMed] [Google Scholar]
  8. MYERS J., KRATZ W. A. Relation between pigment content and photosynthetic characteristics in a blue-green algae. J Gen Physiol. 1955 Sep 20;39(1):11–22. doi: 10.1085/jgp.39.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Neilson A., Rippka R., Kunisawa R. Heterocyst formation and nitrogenase synthesis in Anabaena sp. A kinetic study. Arch Mikrobiol. 1971;76(2):139–150. doi: 10.1007/BF00411788. [DOI] [PubMed] [Google Scholar]
  10. ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]
  11. Simon R. D. Cyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine. Proc Natl Acad Sci U S A. 1971 Feb;68(2):265–267. doi: 10.1073/pnas.68.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TISCHER I. Untersuchungen über die granulären Einschlüsse und das Reduktions-Oxydations-Vermögen der Cyanophyceen. IV. Zellmorphologische und zellphysiologische Studien an Cyanophyceen. Arch Mikrobiol. 1957;27(4):400–428. [PubMed] [Google Scholar]
  13. Wolk C. P. Movement of carbon from vegetative cells to heterocysts in Anabaena cylindrica. J Bacteriol. 1968 Dec;96(6):2138–2143. doi: 10.1128/jb.96.6.2138-2143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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